Effects of growth factors on the differentiation of murine ESC into type II pneumocytes

Samadikuchaksaraei, A. and Bishop, A.E. (2007) Effects of growth factors on the differentiation of murine ESC into type II pneumocytes. Cloning and Stem Cells, 9 (3). pp. 407-416.

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We have previously shown that embryonic stem cells (ESC) can be directed to differentiate into alveolar type II cells by provision of a serum-free medium designed for in vitro maintenance of mature alveolar epithelial cells (small airway growth medium: SAGM), although the target cell yield was low. SAGM comprises a basal serum-free medium (SABM) plus a series of defined supplements. In order to try increase the proportion of pneumocytes in differentiated cultures, we aimed in this study to determine the effects on murine ESC of each of the individual growth factors in SAGM. In accordance with our previous reports, expression of surfactant protein C (SPC) and its mRNA was used to monitor differentiation of type II pneumocytes. Surprisingly, we found that addition of each factor separately to SABM decreased the expression of SPC mRNA when compared with the effect of SABM alone. Thus, it seems that the observed enhancement by SAGM of pneumocyte differentiation from murine ESC can, in fact, be attributed to the provision of a serum-free environment. © Mary Ann Liebert, Inc. 2007.

Item Type: Article
Additional Information: cited By 19
Uncontrolled Keywords: growth factor; messenger RNA; surfactant protein C; messenger RNA; peptide; Sftpc protein, mouse; signal peptide; unclassified drug, animal cell; article; cell differentiation; cell maturation; controlled study; culture medium; embryo; embryonic stem cell; in vitro study; lung alveolus cell type 2; mouse; Murinae; nonhuman; priority journal; protein expression; target cell; animal; cell differentiation; cytology; drug effect; genetics; lung alveolus; metabolism; physiology, Murinae, Animals; Cell Differentiation; Culture Media; Embryonic Stem Cells; Intercellular Signaling Peptides and Proteins; Mice; Peptides; Pulmonary Alveoli; RNA, Messenger
Subjects: QV Pharmacology
Divisions: School of Rehabilitation Sciences
Depositing User: parto mrs bakhtminoo
Date Deposited: 26 Feb 2019 09:01
Last Modified: 26 Feb 2019 09:01
URI: http://eprints.iums.ac.ir/id/eprint/10691

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