In vitro anti-leishmanial activity of methanolic extracts of Calendula officinalis flowers, Datura stramonium seeds, and Salvia officinalis leaves

Nikmehr, B. and Ghaznavi, H. and Rahbar, A. and Sadr, S. and Mehrzadi, S. (2014) In vitro anti-leishmanial activity of methanolic extracts of Calendula officinalis flowers, Datura stramonium seeds, and Salvia officinalis leaves. Chinese Journal of Natural Medicines, 12 (6). pp. 423-427.

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Abstract

Aim: The anti-leishmanial activity of methanolic extracts of Calendula officinalis flowers, Datura stramonium seeds, and Salvia officinalis leaves against extracellular (promastigote) and intracellular (amastigote) forms of Leishmania major were evaluated in this study. Method: In the first stage, promastigote forms of L. major, were treated with different doses of the plant extracts in a 96-well tissue-culture microplate and IC50 values for each extract were measured with colorimetric MTT assay. In the second stage, macrophage cells were infected with L. major promastigotes. Infected macrophages were treated with plant extracts. Then the macrophages were stained with Gimsa and the number of infected macrophages and amastigotes were counted with a light microscope. Results: The results indicated that the plant extracts inhibited the growth of promastigotes and amastigotes of L. major. Inhibitory concentrations (IC50) for promastigote assay were 108.19, 155.15, and 184.32 μg7·mL-1 for C. officinalis flowers, D. stramonium seeds and S. officinalis, respectively. The extracts also reduced the number of amastigotes in macrophage cells from 264 for control group to 88, 97, and 102 for test groups. Although the anti-leishmanial activity of the extracts were not comparable with the standard drug, miltefosine; but they showed significant efficiency in reducing the number of amastigotes in macrophages, in comparison with the control group (P < 0.001). These plant extracts had lower toxicity compared with miltefosine. Conclusion: This study demonstrates the potential efficacy of the methanolic extracts of C. officinalis flowers, D. stramonium seeds, and S. officinalis leaves to control of cutaneous leishmaniasis. © 2014 China Pharmaceutical University.

Item Type: Article
Additional Information: cited By 9
Uncontrolled Keywords: antileishmanial agent; calendula officinalis extract; Datura stramonium extract; miltefosine; plant extract; Salvia officinalis extract; unclassified drug; antiparasitic agent; plant extract, amastigote; antiprotozoal activity; article; Calendula officinalis; controlled study; cytotoxicity; Datura stramonium; in vitro study; Leishmania major; macrophage; nonhuman; promastigote; Salvia officinalis; Calendula; cell line; Datura stramonium; drug effects; flower; leishmaniasis; parasitology; phytotherapy; plant leaf; plant seed; Salvia officinalis, Antiparasitic Agents; Calendula; Cell Line; Datura stramonium; Flowers; In Vitro Techniques; Leishmania major; Leishmaniasis; Macrophages; Phytotherapy; Plant Extracts; Plant Leaves; Salvia officinalis; Seeds
Subjects: QX Parasitology
Depositing User: Ms Roya Vesal Azad
Date Deposited: 04 May 2019 05:14
Last Modified: 04 May 2019 05:14
URI: http://eprints.iums.ac.ir/id/eprint/11775

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