Breast cancer cells imaging by targeting methionine transporters with gadolinium-based nanoprobe

Mehravi, B. and Ardestani, M.S. and Damercheli, M. and Soltanghoraee, H. and Ghanaldarlaki, N. and Alizadeh, A.M. and Oghabian, M.A. and Shirazi, M.S. and Mahernia, S. and Amanlou, M. (2014) Breast cancer cells imaging by targeting methionine transporters with gadolinium-based nanoprobe. Molecular Imaging and Biology, 16 (4). pp. 519-528.

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Abstract

Purpose: Early cancer diagnosis using MRI imaging is of high global interest as a non-invasive and powerful modality. In this study, methionine was conjugated on gadolinium-based mesoporous silica nanospheres to evaluate intra-cellular uptake and its accumulation in human breast cancer cells. Procedures: The contrast agent was synthesized and characterized using different techniques including N2 physisorption, thermal gravimetric analysis, dynamic light scattering, and inductively coupled plasma atomic emission spectroscopy (ICP-AES). The intra-cellular uptake of Gd3+ was measured by ICP-AES, fluorescent microscopy, and flow cytometry. Finally, cellular and tumor MR imaging were performed to determine in vitro and in vivo relaxometry. Results: According to the results, the contrast agents accumulated in tumor cells both in vitro and in vivo. There was no significant cellular toxicity on either normal or cancer cells along with strong intense signal on T1 compared to the unlabeled cells. Conclusions: The results showed that the novel contrast agent could become a useful tool in early detection of cancer. © 2014 World Molecular Imaging Society.

Item Type: Article
Additional Information: cited By 11
Uncontrolled Keywords: gadolinium; gadolinium pentetate meglumine; mesoporous silica nanosphere gadolinium; methionine; methionine mesoporous silica nanosphere gadolinium; nanosphere; nuclear magnetic resonance imaging agent; silicon dioxide; unclassified drug; carrier protein; gadolinium; methionine; silicon dioxide, animal experiment; animal model; article; atomic emission spectrometry; breast cancer; cancer cell; cancer diagnosis; cell viability; controlled study; drug delivery system; drug synthesis; dynamic light scattering; female; flow cytometry; fluorescence microscopy; human; human cell; in vitro study; in vivo study; inductively coupled plasma atomic emission spectroscopy; infrared spectroscopy; light scattering; measurement; mouse; nanoprobe; nonhuman; nuclear magnetic resonance imaging; nuclear magnetic resonance scanner; priority journal; relaxometry; scanning electron microscopy; thermogravimetry; transmission electron microscopy; zeta potential; Breast Neoplasms; cell survival; chemistry; diagnostic imaging; diagnostic use; HEK293 cell line; intracellular space; MCF 7 cell line; metabolism; nuclear magnetic resonance imaging; pathology; porosity; procedures, Breast Neoplasms; Cell Survival; Diagnostic Imaging; Female; Gadolinium; HEK293 Cells; Humans; Intracellular Space; Magnetic Resonance Imaging; MCF-7 Cells; Membrane Transport Proteins; Methionine; Nanospheres; Porosity; Silicon Dioxide
Subjects: WN Radiology. Diagnostic Imaging
Depositing User: Ms Roya Vesal Azad
Date Deposited: 26 Feb 2019 09:19
Last Modified: 26 Feb 2019 09:19
URI: http://eprints.iums.ac.ir/id/eprint/11805

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