Recruiting testicular torsion introduces an azoospermic mouse model for spermatogonial stem cell transplantation

Azizollahi, S. and Aflatoonian, R. and Sedigi-Gilani, M.A. and Jafarabadi, M.A. and Behnam, B. and Azizollahi, G. and Koruji, M. (2014) Recruiting testicular torsion introduces an azoospermic mouse model for spermatogonial stem cell transplantation. Urology Journal, 11 (3). pp. 1648-1655.

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Abstract

Purpose: To investigate the long-term effect of testicular torsion on sperm parameters and testis structure in order to introduce a novel mice azoospermic model for spermatogonial stem cell transplantation. Materials and Methods: Unilateral testicular torsion was created. The animals were divided into two groups each containing 15 mice. They underwent 2 and 4 hours of unilateral testicular ischemia, respectively. All animals in this experiment were aged matched. The experimental (n = 5) groups were studied 2, 4 and 10 weeks after testicular ischemia reperfusion. Moreover, the left testes and epididymis were removed for sperm analysis and for weight and histopathological evaluation. Finally isolated spermatogonial stem cells were transplanted in the testes that underwent 2 hours of ischemia reperfusion, two weeks post-surgery. Results: All the investigated parameters demonstrated a sharp decline at 2, 4 and 10 weeks after testicular torsion, whereas 2-hour ischemia was found to be less injurious in testicular tissue structure. Two months after xenotransplantation, the transplanted cells were localized in the basal of the seminiferous tubules of the recipient ischemic testes. Conclusion: Torsion can cause permanent azoospermia in mouse. Also Testicular torsion 2 weeks after the 2 hours ischemia reperfusion may prove useful for recipient preparation for SSCs transplantation in mouse.

Item Type: Article
Additional Information: cited By 4
Uncontrolled Keywords: adult stem cell; animal; azoospermia; cell proliferation; complication; disease model; epididymis; ischemia; male; mechanical torsion; mouse; pathology; physiology; semen analysis; testis; transplantation; vascularization, Adult Stem Cells; Animals; Azoospermia; Cell Proliferation; Disease Models, Animal; Epididymis; Ischemia; Male; Mice; Semen Analysis; Testis; Torsion, Mechanical
Subjects: WJ Urogenital System
Depositing User: Ms Roya Vesal Azad
Date Deposited: 26 Feb 2019 09:22
Last Modified: 26 Feb 2019 09:22
URI: http://eprints.iums.ac.ir/id/eprint/11814

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