E-selectin gene polymorphisms in Iranian chronic hepatitis B patients

Hajilooi, M. and Alizadeh, A.-H.M. and Ranjbar, M. and Fallahian, F. and Alavian, S.-M. (2007) E-selectin gene polymorphisms in Iranian chronic hepatitis B patients. Hepatitis Monthly, 7 (4). pp. 211-216.

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Background and Aims: The aim of this study was to detect the substitutions Ser128Arg (A128C) and Leu554Phe (T554C) which are responsible for E-selectin polymorphisms in patients with chronic hepatitis B and healthy controls. We investigated possible association of the Ser128Arg and Leu554Phe gene polymorphisms in the E-selectin gene with susceptibility to chronic hepatitis B. Methods: Sixty-three patients with chronic hepatitis B virus infection and 150 healthy subjects were recruited sequentially as they presented to clinic. Classification of chronic hepatitis B virus (HBV)-infected patients was as asymptomatic carrier state (34 patients) and chronic hepatitis B (29 patients). Genomic DNA was isolated from anticoagulated peripheral blood Buffy coat using Miller�s salting-out method. The presence of the E-selectin gene polymorphisms was determined by using polymerase chain reaction amplification refractory mutation system (ARMS). Results: Distribution of E-selectin 128 (A+C-, A+C+, A-C+) genotypes and E-selectin 554 (C+T-, T+C-, C+T+) genotypes were not statistically different in chronic hepatitis B patients and controls (P=0.41 and 0.96, respectively). Also, two groups had no significant difference in distribution of frequencies of allele 128A (P=0.41), 128C (P=0.15), allele 554C (P=0.85), and allele 554T (P=0.76). Carrying of allele 128A (OR=0.58, 95 CI=0.16-2.12), 128C (OR=1.52, 95 CI=0.84-2.74), 554C (OR=1.24, 95 CI=0.12-12.08), and allele 554T (OR=0.88, 95 CI=0.38-2.01) were not risk factors for susceptibility to chronic hepatitis B infection. Conclusions: Carrying E-selectin gene polymorphisms of Ser128Arg and Leu554Phe is not considered risk factor for susceptibility to chronic hepatitis B infection. © 2007, Kowsar Medical Publishing Company. All rights reserved.

Item Type: Article
Additional Information: cited By 4
Uncontrolled Keywords: alanine aminotransferase; aspartate aminotransferase; endothelial leukocyte adhesion molecule 1; genomic DNA, adult; Article; chronic hepatitis B; controlled study; correlation analysis; disease predisposition; DNA polymorphism; E selectin gene; female; gene; genetic susceptibility; genotyping technique; hepatobiliary parameters; histologic classification index; human; Leu554Phe gene; major clinical study; male; polymerase chain reaction; risk factor; Ser128Arg gene
Subjects: WC Communicable Diseases
Divisions: School of Rehabilitation Sciences
Depositing User: parto mrs bakhtminoo
Date Deposited: 26 Feb 2019 11:49
Last Modified: 26 Feb 2019 11:49
URI: http://eprints.iums.ac.ir/id/eprint/12311

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