Human Papillomavirus Type 16 Integration Analysis by Real-time PCR Assay in Associated Cancers

Karbalaie Niya, M.H. and Keyvani, H. and Safarnezhad Tameshkel, F. and Salehi-Vaziri, M. and Teaghinezhad-S, S. and Bokharaei Salim, F. and Monavari, S.H.R. and Javanmard, D. (2018) Human Papillomavirus Type 16 Integration Analysis by Real-time PCR Assay in Associated Cancers. Translational Oncology, 11 (3). pp. 593-598.

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Human papillomavirus (HPV) is a common viral infection worldwide associated with a variety of cancers. The integration of the HPV genome in these patients causes chromosomal instability and triggers carcinogenesis. The aim of this study was to investigate the HPV-16 genome physical status in four major cancers related to HPV infection. Formalin-fixed paraffin-embedded blocks from our previous projects on head and neck, colorectal, penile, and cervical cancers were collected, and HPV-16�positive specimens were used for further analysis. The DNA extraction copy number of E2 and E7 genes was calculated by qualitative real-time PCR method. Serially diluted standards that were cloned in PUC57 plasmid were used. Standard curve and melting curve analysis was used for quantification. Of the 672 specimens studied, 76 (11.3) were HPV-16 positive. We found that 35.6 (16/45) were integrated. Statistical analysis showed that there were significant correlations between integration of HPV-16 and cervical cancer end-stage carcinogenesis (P <.0001), episomal form, and ASCUS lesions (P =.045). Significant correlation in penile cancer patients was seen between the episomal form and high-grade cancer stage (P =.037). Integration is a major factor in the carcinogenesis mechanism of HPV and has different prevalence in various cancers with a higher rate in progression except in penile cancer. © 2018

Item Type: Article
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Date Deposited: 05 Aug 2018 05:48
Last Modified: 05 Aug 2018 05:48

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