Noscapine protects OLN-93 oligodendrocytes from ischemia-reperfusion damage: Calcium and nitric oxide involvement

Nadjafi, S. and Ebrahimi, S.-A. and Rahbar-Roshandel, N. (2015) Noscapine protects OLN-93 oligodendrocytes from ischemia-reperfusion damage: Calcium and nitric oxide involvement. Acta Physiologica Hungarica, 102 (4). pp. 351-362.

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Abstract

This study was carried out to evaluate the effects of noscapine, a benzylisoquinoline alkaloid from opium poppy, on oligodendrocyte during ischemia/reperfusion-induced excitotoxic injury. Changes in intracellular calcium levels due to chemical ischemia and nitric oxide (NO) production during ischemia/reperfusion were evaluated as the hallmarks of ischemia-derived excitotoxic event. OLN-93 cell line (a permanent immature rat oligodendrocyte) was used as a model of oligodendrocyte. 30- or 60-minute-oxygen-glucose deprivation/24 hours reperfusion were used to induce excitotoxicity. MTT (3-4,5-Dimethylthiazol-2-yl-2,5-diphenyl-tetrazolium bromide) assay was used to evaluate cell viability. Ratiometric fluorescence microscopy using Ca2+-sensitive indicator Fura-2/AM was utilized to assess intracellular calcium levels. NO production was evaluated by Griess method. Noscapine (4 μM) significantly attenuated intracellular Ca2+ elevation (P < 0.001). Also, noscapine significantly decreased NO production during a 30-minute oxygen-glucose deprivation/reperfusion (P < 0.01). The inhibitory effect of noscapine (4 μM) on intracellular Ca2+ was greater than ionotropic glutamate receptors antagonists. Noscapine is protective against ischemia/reperfusion-induced excitotoxic injury in OLN-93 oligodendrocyte. This protective effect seems to be related to attenuation of intracellular Ca2+ overload and NO production. © 2015 Akadémiai Kiadó, Budapest.

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: 3 (4,5 dimethyl 2 thiazolyl) 2,5 diphenyltetrazolium bromide; 6 nitro 7 sulfamoylbenzofquinoxaline 2,3 dione; calcium; dizocilpine; fura 2 acetoxymethyl ester; glucose; n(g) nitroarginine methyl ester; nitric oxide; noscapine; oxygen, animal cell; animal experiment; animal model; Article; brain ischemia; brain protection; calcium cell level; cell damage; cell viability; clinical assessment; clinical evaluation; controlled study; drug effect; excitotoxicity; fluorescence microscopy; MTT assay; nonhuman; oligodendroglia; outcome assessment; rat; reperfusion injury; sensitivity analysis
Depositing User: EPrints System Admin
Date Deposited: 19 Jul 2017 10:13
Last Modified: 19 Jul 2017 10:13
URI: http://eprints.iums.ac.ir/id/eprint/25

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