Neuroprotective effect of noscapine on cerebral oxygen-glucose deprivation injury

Vahabzadeh, G. and Rahbar-Roshandel, N. and Ebrahimi, S.-A. and Mahmoudian, M. (2015) Neuroprotective effect of noscapine on cerebral oxygen-glucose deprivation injury. Pharmacological Reports, 67 (2). pp. 281-288.

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Methods Cells were transferred to glucose-free DMEM and were exposed to hypoxia in a small anaerobic chamber. Cell viability and nitric oxide production were evaluated by MTT assay and the Griess method, respectively. Results The neurotoxicities produced by all three hypoxia durations tested were significantly inhibited by 0.5 μM noscapine. Increasing noscapine concentration up to 2.5 μM produced a concentration-dependent inhibition of neurotoxicity. Pretreatment of cells with MK-801 (10 μM), a non-competitive NMDA antagonist, and nimodipine (10 nM), an L-type Ca2+ channel blockers, increased cell viability after 30 min OGD, while the application of NBQX (30 μM), a selective AMPA-kainate receptor antagonist partially attenuated cell injury. Subsequently, cells treated with noscapine in the presence of thapsigargin (1 μM), an inhibitor of endoplasmic reticulum Ca2+ ATPases. After 60 min OGD, noscapine could inhibit the cell damage induced by thapsigargin. However, noscapine could not reduce cell damage induced by 240 min OGD in the presence of thapsigargin. Noscapine attenuated nitric oxide (NO) production in cortical neurons after 30 min OGD. Conclusions We concluded that noscapine had a neuroprotective effect, which could be due to its interference with multiple targets in the excitotoxicity process. These effects could be mediated partially by a decrease in NO production and the modulation of intracellular calcium levels. Background The present study aims to investigate the effect of noscapine (0.5-2.5 μM), an alkaloid from the opium poppy, on primary murine fetal cortical neurons exposed to oxygen-glucose deprivation (OGD), an in vitro model of ischemia. © 2014 Institute of Pharmacology, Polish Academy of Sciences. Published by Elsevier Urban & Partner Sp. z o.o. All rights reserved.

Item Type: Article
Additional Information: cited By 1
Uncontrolled Keywords: 6 nitro 7 sulfamoylbenzofquinoxaline 2,3 dione; dizocilpine; glucose; n(g) nitroarginine methyl ester; neuroprotective agent; nimodipine; nitric oxide; nitrite; noscapine; oxygen; thapsigargin; 2,3-dioxo-6-nitro-7-sulfamoylbenzo(f)quinoxaline; dizocilpine maleate; glucose; neuroprotective agent; nitric oxide; noscapine; quinoxaline derivative; thapsigargin, animal cell; animal experiment; Article; brain cell culture; brain injury; calcium cell level; cell damage; cell hypoxia; cell proliferation; cell viability; concentration response; controlled study; embryo; excitotoxicity; female; fetus; in vitro study; intracellular signaling; mouse; MTT assay; neuroprotection; neurotoxicity; nonhuman; oxygen glucose deprivation; animal; anoxia; brain ischemia; cell survival; deficiency; dose response; drug effects; metabolism; nerve cell; pathology; primary cell culture, Animals; Anoxia; Brain Ischemia; Cell Survival; Dizocilpine Maleate; Dose-Response Relationship, Drug; Glucose; Mice; Neurons; Neuroprotective Agents; NG-Nitroarginine Methyl Ester; Nimodipine; Nitric Oxide; Noscapine; Primary Cell Culture; Quinoxalines; Thapsigargin
Depositing User: EPrints System Admin
Date Deposited: 19 Jul 2017 10:13
Last Modified: 19 Jul 2017 10:13

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