Effect of berberine on nitric oxide production during oxygen-glucose deprivation/reperfusion in OLN-93 oligodendrocytes

Nadjafi, S. and Ebrahimi, S.-A. and Rahbar-Roshandel, N. (2014) Effect of berberine on nitric oxide production during oxygen-glucose deprivation/reperfusion in OLN-93 oligodendrocytes. Pakistan Journal of Biological Sciences, 17 (11). pp. 1185-1189.

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Abstract

In this study, the effect of berberine, an isoquinoline alkaloid isolated from Coptidis rhizoma, on Nitric Oxide (NO) production, as a possible involved factor, during excitotoxic injury in oligodendroglial cells were evaluated. The overactivation of ionotropic glutamate receptors which is known as the excitotoxicity, is an important phenomenon because of the contribution in acute injury to the central nervous system, chronic neurodegenerative disorders, oligodendrocyte loss and demyelinating diseases as Multiple Sclerosis (MS). Intracellular Ca2+ overload, have a key role during excitotoxic injury and such increase in cytoplasmic Ca2+ triggers a series of events such as production of NO that end to cell death. Previous report showed the protective effects of berberine on ischemic-induced excitotoxic insult in oligodendrocytes. Hereby, we intended to know if the NO production could be associated with oxygen-glucose deprivation/reperfusion-induced excitotoxic damage in oligodendrocyte; moreover, the alteration of NO production could be considered as an involved mechanism for protective effect of berberine in such condition. Therefore, the effect of berberine (2 µM) on NO production during oxygen-glucose deprivation/24 h reperfusion in oligodendrocytes were examined. The OLN-93 cell line (a permanent immature rat oligodendrocyte) was used as a model of oligodendrocyte. Thirty minutes-oxygen-glucose deprivation/24 h reperfusion was used to induce excitotoxicity. NO production was evaluated by Griess method. Our results demonstrated that berberine (2 µcM) significantly decreased NO production during 30 min oxygen-glucose deprivation/reperfus ion. It seems that blockade of NO production by berberine may also participate in oligodendroglial cell protection against oxygen-glucose deprivation/ reperfusion-induced insult. © 2014 Asian Network for Scientific Information.

Item Type: Article
Additional Information: cited By 2
Uncontrolled Keywords: amino acid receptor blocking agent; berberine; enzyme inhibitor; glucose; n methyl dextro aspartic acid receptor; neuroprotective agent; nitric oxide; nitric oxide synthase; oxygen, animal; antagonists and inhibitors; cell hypoxia; cell protection; deficiency; down regulation; drug effects; metabolism; oligodendroglia; rat; time; transformed cell line, Animals; Berberine; Cell Hypoxia; Cell Line, Transformed; Cytoprotection; Down-Regulation; Enzyme Inhibitors; Excitatory Amino Acid Antagonists; Glucose; Neuroprotective Agents; Nitric Oxide; Nitric Oxide Synthase; Oligodendroglia; Oxygen; Rats; Receptors, N-Methyl-D-Aspartate; Time Factors
Depositing User: EPrints System Admin
Date Deposited: 19 Jul 2017 10:13
Last Modified: 19 Jul 2017 10:13
URI: http://eprints.iums.ac.ir/id/eprint/33

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