Immunoreactivity analysis of Toxoplasma gondii recombinant antigen rSAG3 in sera from immunized BALB/c mice and tox-oplasmosis patients

Motazedian, M.H. and Kazemi, B. and Shahriari, B. and Bandehpour, M. and Khanaliha, K. (2016) Immunoreactivity analysis of Toxoplasma gondii recombinant antigen rSAG3 in sera from immunized BALB/c mice and tox-oplasmosis patients. Iranian Journal of Public Health, 45 (7). pp. 911-916.

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Abstract

Background: The coccidian protozoa Toxoplasma gondii is an obligate intracellular parasite of humans and other warm-blooded animals. Diagnosis of toxoplasmosis is of considerable medical importance for human, especially pregnant women and immunocompromised individuals. The apply of an Escherichia coli recombinant antigen(s) would be signifi-cantly useful in developing standardization of the diagnostic tests and reducing their costs. In this study, immunoreac-tivity of recombinant SAG3 against sera from immunized mice and human anti-T. gondii IgG positive patients was evaluated by western-blotting and enzyme immunoassay (EIA) in Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences in 2013. Methods: Three inbreed BALB/c female mice were obtained. Two mice were injected with rSAG3 and one was re-mained untreated, as control. Sera from immunized mice and also pooled sera from IgG positive toxoplasmosis cases were evaluated with western-blotting. IgG antibody responses to recombinant SAG3 was measured by indirect ELISA against the negative control group. Results: The rSAG3 protein reacted with sera of immunized mice and sera from patients with anti-Toxoplasma IgG antibodies in western-blot analysis. The result of ELISA showed that, there was marked differences in the absorbance values between the recombinant SAG3 immunized mice and control group. Conclusion: The rSAG3 showed IgG reactivity with sera from immunized mice and anti-Toxoplasma IgG patients. © 2016, Iranian Journal of Public Health. All rights reserved.

Item Type: Article
Additional Information: cited By 0
Subjects: WC Communicable Diseases
QV Pharmacology
Depositing User: eprints admin
Date Deposited: 07 Jul 2018 03:44
Last Modified: 16 Oct 2019 08:07
URI: http://eprints.iums.ac.ir/id/eprint/3466

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