Deciphering the binding mode of dinitramine herbicide to ct-DNA, a thermodynamic discussion

Daneshmehr, M.-A. and Ahmadi, F. and Ahmadi, B. and Shakiba, E. (2016) Deciphering the binding mode of dinitramine herbicide to ct-DNA, a thermodynamic discussion. Food and Agricultural Immunology, 27 (1). pp. 23-39.

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Abstract

Dinitramine is a herbicide that has been used to control annual grasses and broadleaf weeds in cotton and soybeans in Iran. In this study, the electrochemical behavior of dinitramine was studied by cyclic voltammetry (CV) and differential pulse voltammetry (DPV) methods. The interaction of dinitramine with ct-DNA was evaluated by CV, competitive fluorescence, UV-Vis spectroscopy, FT-IR spectroscopy, and viscosity titration. In addition, the thermodynamic parameters of DIN�DNA complex were calculated by spectrophotometric titration. The values of �Hbin., �Sbin., and �Gbin. (T = 290.65 K) of the DIN�DNA complex were +39.25 kJ mol�1, +215.71 J mol�1, and �23.45 KJ mol�1, respectively. These data revealed that the endothermic binding has its origin in the hydrophobic interactions. Also the high positive �Sbin was explained according to the DIN structure that optimized by mechanical quantum calculations. However, all data showed that the major groove binding between DIN and ct-DNA is more predominant than other binding modes. © 2015 Taylor & Francis.

Item Type: Article
Additional Information: cited By 1
Subjects: QU Biochemistry. Cell Biology and Genetics
QV Pharmacology
Depositing User: eprints admin
Date Deposited: 04 Jul 2018 04:54
Last Modified: 04 Nov 2019 11:44
URI: http://eprints.iums.ac.ir/id/eprint/3999

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