5-HT2A serotonin receptor density in adult male rats' hippocampus after morphine-based conditioned place preference

Mohammadi, R. and Jahanshahi, M. and Jameie, S.B. (2016) 5-HT2A serotonin receptor density in adult male rats' hippocampus after morphine-based conditioned place preference. Basic and Clinical Neuroscience, 7 (3). pp. 249-258.

[img] Text

Download (926kB)
Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....


Introduction: A close interaction exists between the brain opioid and serotonin (5-HT) neurotransmitter systems. Brain neurotransmitter 5-HT plays an important role in the regulation of reward-related processing. However, a few studies have investigated the potential role of 5-HT2A receptors in this behavior. Therefore, the aim of the present study was to assess the influence of morphine and Conditioned Place Preference (CPP) on the density of 5-HT2A receptor in neurons of rat hippocampal formation. Methods: Morphine (10 mg/kg, IP) was injected in male Wistar rats for 7 consecutive days (intervention group), but control rats received just normal saline (1 mL/kg, IP). We used a hotplate test of analgesia to assess induction of tolerance to analgesic effects of morphine on days 1 and 8 of injections. Later, two groups of rats were sacrificed one day after 7 days of injections, their whole brains removed, and the striatum and PFC immediately dissected. Then, the NR1 gene expression was examined with a semi-quantitative RT-PCR method. Results: Our data showed that the maximum response was obtained with 2.5 mg/kg of morphine. The density of 5-HT2A receptor in different areas of the hippocampus increased significantly at sham-morphine and CPP groups (P<0.05). On the other hand, the CPP groups had more 5-HT2A receptors than sham-morphine groups and also the sham-morphine groups had more 5-HT2A receptors than the control groups. Conclusion: We concluded that the phenomenon of conditioned place preference induced by morphine can cause a significant increase in the number of serotonin 5-HT2A receptors in neurons of all areas of hippocampus.

Item Type: Article
Additional Information: cited By 2
Subjects: WL Nervous System
QV Pharmacology
Depositing User: eprints admin
Date Deposited: 02 Jul 2018 10:21
Last Modified: 10 Nov 2019 10:17
URI: http://eprints.iums.ac.ir/id/eprint/4150

Actions (login required)

View Item View Item