Interaction of A-240T and A2350G related genotypes of angiotensin- converting enzyme (ACE) is associated with decreased serum ACE activity and blood pressure in a healthy Iranian population

Firouzabadi, N. and Tajik, N. and Shafiei, M. and Ebrahimi, S.A. and Bakhshandeh, H. (2011) Interaction of A-240T and A2350G related genotypes of angiotensin- converting enzyme (ACE) is associated with decreased serum ACE activity and blood pressure in a healthy Iranian population. European Journal of Pharmacology, 668 (1-2). pp. 241-247.

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Abstract

Most of renin-angiotensin system (RAS) gene polymorphisms have not yet been studied in the Iranian population. In the present study, the frequencies of common polymorphisms in the RAS genes, including angiotensin-converting enzyme (ACE) insertion/deletion (I/D) and three single-nucleotide polymorphisms (SNPs), i.e., A-240T, T-93C and A2350G, angiotensinogen M235T, angiotensin II receptor type 1 A1166C and angiotensin II receptor type 2 C3123A variants were determined in DNAs extracted from venous blood of 104 healthy Iranian volunteers. Genotyping was performed by PCR-RFLP method. Serum ACE activity was also assayed using reverse phase HPLC. Combined polymorphisms of TT (A-240T) and GG (A2350G) was significantly associated with decreased serum ACE activity (P = 0.042) and decreased diastolic blood pressure (P = 0.040). The angiotensin II receptor type 1 A1166C polymorphism (CC genotype) showed a significant association with declined diastolic blood pressure (P = 0.028). Serum ACE activity was significantly higher in men compared to women (P = 0.033). ACE activity also showed a direct association with diastolic blood pressure (P < 0.001). No association was obtained among each single polymorphism with body mass index (BMI), fasting blood sugar (FBS), lipid profile and ACE activity. In conclusion, combined polymorphisms of A-240T and A2350G seem to affect serum ACE level as well as diastolic blood pressure in our study population. However, it also might be hypothesized that they are in strong linkage disequilibrium with other functional mutations not studied yet. Our findings revealed that gene interactions can play an important role in various biological conditions. © 2011 Elsevier B.V.

Item Type: Article
Additional Information: cited By 12
Uncontrolled Keywords: angiotensin 1 receptor; angiotensin 2 receptor; angiotensinogen; dipeptidyl carboxypeptidase; genomic DNA; glucose; lipid, article; body mass; diastolic blood pressure; DNA extraction; DNA polymorphism; enzyme activity; female; gene interaction; gene linkage disequilibrium; genotype; glucose blood level; human; indel mutation; Iran; male; normal human; polymerase chain reaction; priority journal; protein variant; renin angiotensin aldosterone system; restriction fragment length polymorphism; reversed phase high performance liquid chromatography; sex difference; single nucleotide polymorphism; venous blood, Blood Pressure; Female; Gene Frequency; Genotype; Health; Humans; Iran; Male; Middle Aged; Peptidyl-Dipeptidase A; Polymorphism, Single Nucleotide
Depositing User: EPrints System Admin
Date Deposited: 19 Jul 2017 10:13
Last Modified: 19 Jul 2017 10:13
URI: http://eprints.iums.ac.ir/id/eprint/44

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