Interaction between ketoconazole, amphotericin B and terbinafin and three diazenumdiolates in concomitant uses against some fugal species

Falahati, M. and Shabani, M. and Rodaki, M.M.A. and Jahaniani, F. and Bagheri, K.P. and Ebrahimi, S.A. (2006) Interaction between ketoconazole, amphotericin B and terbinafin and three diazenumdiolates in concomitant uses against some fugal species. Daru, 14 (2). pp. 87-92.

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A checkerboard broth microdilution method was performed to investigate the in vitro antifungal activities of three diazeniumdiolates derivatives (DETA/NO, DPTA/NO, DEA/NO) alone and in combination with ketoconazole, amphotricin B or terbinafine against five Candida species, Cryptococcus neoformance and four dermatophyte strains. MICs and MLCs were recorded, and synergy was calculated by using fractional inhibitory and fractional lethal concentration index. DETA/NO with a half-life of 57h at 25°C showed antifungal activity against all tested dermatophyte species (MIC 0.150 to 2.5mg/ml), DPTA/NO with a half life of 3h at 37°C showed antifungal activity against five species of Candida and Cryptococcus neoformans, and DEA/NO with a half life of 2 min at 37°C and 16 min at 25° C did not show antifungal activity against tested strains. Combinations of DPTA-NO with either ketoconazole or amphotericin B were either synergistic or indifferent for all tested strain of Candida and Cryptococcus neoformance. DETA/NO was unable to enhance the antifungal activity of terbinafine against dermatophyte strains. Even where no synergistic activity was achieved, there was still a decrease in the MIC of one or both drugs which were used in combination. Antagonism was observed between terbinafine and DETA-NO against Trichophyton rubrum. Our result suggests that DETA/NO and DPTA/NO may be useful for development of new therapeutic strategies for treatment of dermatophyte and Candida infections. Clinical studies are warranted to elucidate the potential utility of these combination therapies.

Item Type: Article
Additional Information: cited By 2
Uncontrolled Keywords: 1 n(2 aminoethyle)n(2 aminoethyle)aminodiazen 1 ium 1,2 diolate; 3,3 (hydroxynitrosohydrazino)bis 1 propanamine; amphotericin B; antifungal agent; ketoconazole; nitric oxide donor; sodium 1 n,n diethlamineo; terbinafine; unclassified drug, antifungal activity; article; Candida; Cryptococcus neoformans; dermatophyte; drug antagonism; drug half life; drug potentiation; fungus; in vitro study; LC 50; minimum inhibitory concentration; nonhuman
Depositing User: EPrints System Admin
Date Deposited: 19 Jul 2017 10:13
Last Modified: 19 Jul 2017 10:13

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