Antiviral effects of Lactobacillus crispatus against HSV-2 in mammalian cell lines

Mousavi, E. and Makvandi, M. and Teimoori, A. and Ataei, A. and Ghafari, S. and Samarbaf-Zadeh, A. (2018) Antiviral effects of Lactobacillus crispatus against HSV-2 in mammalian cell lines. Journal of the Chinese Medical Association, 81 (3). pp. 262-267.

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Background: Herpes simplex virus type 2 (HSV-2) infectious disease is one of the most common viral sexually transmitted diseases. As regards, vaginal lactobacilli play an important role in protecting host against the urogenital pathogens; here we assessed the potential antiviral activity of Lactobacillus crispatus against HSV-2 infection in vitro. Methods: Both Vero and HeLa cell lines were treated by L. crispatus before, during and after HSV-2 infection. The pre-incubation assay was also performed for the evaluating of virus adsorption by L. crispatus. Virus titer reduction in each stage was determined by a plaque reduction assay. Results: L. crispatus significantly decreased the infectivity of the HSV-2 in initial steps on both cell lines; however, no significant inhibition was ascertained during adsorption and multiplication process. The lactobacilli adhere on Vero cells two-fold stronger than HeLa and subsequently protect the Vero cells nearly 2.5 fold higher than HeLa cell against the virion. Co-incubation of HSV-2 with bacterial cells prior to virus inoculation significantly decreased the virus titer. Conclusion: L. crispatus appears to inhibit the entry of the virus into cells by trapping HSV-2 particles. In addition, formation of L. crispatus microcolonies in the cell surface could block HSV-2 receptors and prevent viral entry to cells in initial infection steps. © 2017

Item Type: Article
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Depositing User: eprints admin
Date Deposited: 05 Aug 2018 04:51
Last Modified: 05 Aug 2018 04:51

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