Clinical Grade Human Adipose Tissue-Derived Mesenchymal Stem Cell Banking

Larijani, B. and Aghayan, H. and Goodarzi, P. and Mohamadi-Jahani, F. and Norouzi-Javidan, A. and Dehpour, A.R. and Fallahzadeh, K. and Azam Sayahpour, F. and Bidaki, K. and Arjmand, B. (2015) Clinical Grade Human Adipose Tissue-Derived Mesenchymal Stem Cell Banking. Acta medica Iranica, 53 (9). pp. 540-546.

[img] Text
Clinical Grade Human Adipose Tissue-Derived Mesenchymal Stem Cell Banking.pdf

Download (406kB)
Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

In this study, our aim was to produce a generation of GMP-grade adipose tissue-derived mesenchymal stem cells for clinical applications. According to our results, we fulfill to establish consistent and also reproducible current good manufacturing practice (cGMP) compliant adipose tissue-derived mesenchymal stem cells from five female donors. The isolated cells were cultured in DMEM supplemented with 10 fetal bovine serum and characterized by standard methods. Moreover, karyotyping was performed to evaluate chromosomal stability. Mean of donors' age was 47.6 ± 8.29 year, mean of cell viability was 95.6 ± 1.51, and cell count was between 9�106 and 14�106 per microliter with the mean of 12.2�106 ± 2863564.21 per microliter. The main aim of this project was demonstrating the feasibility of cGMP-compliant and clinical grade adipose tissue-derived mesenchymal stem cells preparation and banking for clinical cell transplantation trials.

Item Type: Article
Additional Information: cited By 1
Subjects: QU Biochemistry. Cell Biology and Genetics
Depositing User: eprints admin
Date Deposited: 01 Jul 2018 07:08
Last Modified: 21 Jan 2020 11:13
URI: http://eprints.iums.ac.ir/id/eprint/5695

Actions (login required)

View Item View Item