Emergence of fosfomycin resistance among isolates of Escherichia coli harboring extended-spectrum and ampc β-lactamases

Bahramian, A. and Eslami, G. and Hashemi, A. and Tabibi, A. and Heidary, M. (2018) Emergence of fosfomycin resistance among isolates of Escherichia coli harboring extended-spectrum and ampc β-lactamases. Acta Microbiologica et Immunologica Hungarica, 65 (1). pp. 15-25.

Full text not available from this repository.
Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....


Urinary tract infection (UTI) is a common type of infectious disease globally. The aim of this study was to detect the frequency of fosA3 and fosC2 genes in extended-spectrum β-lactamases (ESBL) and blaDHA, blaCMY-2, and blaCMY-42 genes in AmpC β-lactamases-producing isolates of Escherichia coli. In total, 120 isolates of E. coli were collected from three teaching hospitals between March 2014 and February 2015. Antibiotic susceptibility tests were carried out by disk diffusion method. The presence of blaCMY-2, blaCMY-42, blaDHA, fosA3, and fosC2 genes was detected by polymerase chain reaction (PCR) and sequencing. Of the 120 strains, 92 (76.6) were identified as ESBL producers, 30 (25) were determined as AmpC β-lactamase producers, and 24 (20) had both ESBL and AmpC β-lactamase enzymes. Imipenem, fosfomycin, and nitrofurantoin had the best effect against isolates of E. coli. PCR assay demonstrated that the frequency of blaCMY-2, blaCMY-42, and blaDHA genes among AmpC β-lactamases-producing strains were 39, 1, and 17.5, respectively. This study reports the first detection of fosfomycin resistance in Iran. This study indicated the increasing prevalence of UTI isolates of E. coli-harboring ESBL and AmpC β-lactamases genes in Iran. Therefore, due to the high rate of blaDHA and blaCMY genes and emergence of fosfomycin-resistant E. coli isolates, we recommend continuous monitoring of antibiotic resistance as well as attention to guidelines of infection controls. © 2017 Akadémiai Kiadó, Budapest.

Item Type: Article
Additional Information: cited By 1
Uncontrolled Keywords: amikacin; ampicillin; bacterial protein; beta lactamase AmpC; cefotaxime; cefoxitin; cefpodoxime; ceftazidime; ceftriaxone; ciprofloxacin; cotrimoxazole; fosfomycin; gentamicin; imipenem; levofloxacin; nitrofurantoin; protein bla cmy 2; protein bla cmy 42; protein bla dha; protein fosa3; protein fosc2; tobramycin; unclassified drug; AmpC beta-lactamases; antiinfective agent; bacterial protein; beta lactamase; Escherichia coli protein; fosfomycin, adolescent; adult; aged; antibiotic resistance; antibiotic sensitivity; Article; bacterial strain; bacterium isolate; child; controlled study; disk diffusion; extended spectrum beta lactamase producing Escherichia coli; female; gene frequency; human; Iran; major clinical study; male; nucleotide sequence; polymerase chain reaction; prevalence; urinary tract infection; drug effect; enzymology; Escherichia coli; Escherichia coli infection; genetics; infant; isolation and purification; metabolism; microbial sensitivity test; microbiology; middle aged; preschool child; urinary tract infection; young adult, Adolescent; Adult; Aged; Anti-Bacterial Agents; Bacterial Proteins; beta-Lactamases; Child; Child, Preschool; Drug Resistance, Bacterial; Escherichia coli; Escherichia coli Infections; Escherichia coli Proteins; Female; Fosfomycin; Humans; Infant; Male; Microbial Sensitivity Tests; Middle Aged; Urinary Tract Infections; Young Adult
Subjects: QV Pharmacology
Depositing User: eprints admin
Date Deposited: 31 Dec 2018 07:32
Last Modified: 31 Dec 2018 07:32
URI: http://eprints.iums.ac.ir/id/eprint/6082

Actions (login required)

View Item View Item