An in vitro evaluation of human DNA topoisomerase I inhibition by Peganum harmala L. seeds extract and its β-carboline alkaloids

Sobhani, A.M. and Ebrahimi, S.-A. and Mahmoudian, M. (2002) An in vitro evaluation of human DNA topoisomerase I inhibition by Peganum harmala L. seeds extract and its β-carboline alkaloids. Journal of Pharmacy and Pharmaceutical Sciences, 5 (1). pp. 19-23.

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Abstract

Purpose. Peganum harmala L. (Zygophyllaceae) seeds extract is one of the main components of an ethnobotanical preparation used in the treatment of neoplasms in Iran. Cytotoxic effects of P. harmala extract on cancerous cell-lines have been reported before. β-carbolines like harmaline and harmine are the major alkaloids present in the seeds of the P. harmala. Considering reports concerning DNA topoisomerase inhibition by other β-carbolines like harmane, we have used DNA relaxation assays to investigate topoisomerase I inhibitory activity of P. harmala seeds extract and its β-carboline alkaloids to further inspecting the mechanism of its cytotoxic activity. Methods. Harmine and harmaline contents of the extract were determined using an HPTLC method. DNA topoisomerase I enzyme needed for investigating inhibitory effect of the compounds using DNA relaxation assay, was partially purified from the human placenta. DNA relaxation assay is based on the conversion of a supercoiled plasmid substrate to its relaxed form by the catalytic activity of the enzyme. The supercoiled substrate and its relaxed product can be easily distinguished using agarose gel electrophoresis, since the relaxed topological isomers of DNA migrate more slowly than supercoiled species. Results. Using HPTLC method, it was found that each gram of dried extract contained 55.5 and 79.0 mg of harmine and harmaline respectively. In the DNA relaxation assay, order of potency was harmine > harmane > harmaline > extract. The most active compound was harmine with IC50 value of 13.5 ± 1.7 μg/ml. Conclusions. Our in vitro findings demonstrate that P. harmala seeds extract do inhibit human DNA topoisomerase I and based on the results of HPTLC analysis, it appears that the biological activity of the extract can be explained by its β- carboline content.

Item Type: Article
Additional Information: cited By 106
Uncontrolled Keywords: alkaloid; beta carboline derivative; DNA topoisomerase; harmaline; harman; harmine; Peganum harmala extract; plasmid DNA; teniposide, agar gel electrophoresis; antineoplastic activity; article; cancer cell culture; controlled study; DNA purification; DNA supercoiling; drug cytotoxicity; drug effect; drug mechanism; drug potency; drug screening; enzyme degradation; enzyme inhibition; ethnopharmacology; high performance thin layer chromatography; human; human tissue; IC 50; in vitro study; placenta; plant seed, Chromatography, High Pressure Liquid; DNA; DNA Topoisomerases, Type I; Dose-Response Relationship, Drug; Drug Evaluation, Preclinical; Enzyme Inhibitors; Humans; Placenta; Plant Extracts; Seeds; Zygophyllaceae
Depositing User: EPrints System Admin
Date Deposited: 19 Jul 2017 10:13
Last Modified: 19 Jul 2017 10:13
URI: http://eprints.iums.ac.ir/id/eprint/62

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