Folic acid-cysteamine modified gold nanoparticle as a nanoprobe for targeted computed tomography imaging of cancer cells

Khademi, S. and Sarkar, S. and Shakeri-Zadeh, A. and Attaran, N. and Kharrazi, S. and Ay, M.R. and Ghadiri, H. (2018) Folic acid-cysteamine modified gold nanoparticle as a nanoprobe for targeted computed tomography imaging of cancer cells. Materials Science and Engineering C, 89. pp. 182-193.

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Abstract

Development of various cost-effective multifunctional nanoprobes for efficient targeted molecular imaging of tumors remains a great challenge in medicine. Herein, we report a simple method of forming folic acid-targeted multifunctional gold nanoparticles via cost-effective cysteamine as a template for tumor molecular computed tomography (CT) imaging technique. The formed multifunctional cysteamine-folic acid conjugated gold nanoparticles (FA-Cys-AuNPs) were characterized via different techniques. Colony assay, hematoxylin and eosin (H&E), MTT, and flow cytometry analysis were used to evaluate the cytocompatibility of the particles. We showed that the formed FA-Cys-AuNPs with an Au core size of ~15 nm are non-cytotoxic in a given concentration range and revealed greater X-ray attenuation intensity than iodine-based contrast agent under the same concentration of the active element. At 80 kVp, FA-Cys-AuNPs enable 1.77�times greater contrast per unit mass compared with iodine at a concentration of 2000 μg/ml, and importantly, the developed FA-Cys-AuNPs can be used as a contrast media for targeted CT imaging of folic acid receptor-expressing cancer cells in vitro. CT values of the targeted cells were 2-times higher than that of non-targeted cells at 80 kVp. These findings propose that the designed FA-Cys-AuNPs can be used as a promising contrast agent for molecular CT imaging. This data can be also considered for the application of gold nanostructures in radiation dose enhancement where nanoparticles with high X-ray attenuation are applied. © 2018 Elsevier B.V.

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: Cells; Contrast media; Cost effectiveness; Cytology; Diseases; Fiber optic sensors; Gold nanoparticles; Iodine; Metal nanoparticles; Molecular imaging; Nanoprobes; Organic acids; Tumors; X rays, Cancer; Concentration ranges; CT imaging; Cytocompatibility; Flow cytometry analysis; Folic acids; Gold nanostructures; Multifunctional nanoprobes, Computerized tomography, biomaterial; contrast medium; folic acid; gold; mercaptamine; metal nanoparticle, cell cycle checkpoint; cell survival; chemistry; diagnostic imaging; drug effect; human; infrared spectroscopy; neoplasm; procedures; spectrophotometry; transmission electron microscopy; tumor cell line; x-ray computed tomography, Biocompatible Materials; Cell Cycle Checkpoints; Cell Line, Tumor; Cell Survival; Contrast Media; Cysteamine; Folic Acid; Gold; Humans; Metal Nanoparticles; Microscopy, Electron, Transmission; Neoplasms; Spectrophotometry; Spectroscopy, Fourier Transform Infrared; Tomography, X-Ray Computed
Subjects: WN Radiology. Diagnostic Imaging
QV Pharmacology
Depositing User: eprints admin
Date Deposited: 24 Dec 2018 07:37
Last Modified: 24 Dec 2018 07:37
URI: http://eprints.iums.ac.ir/id/eprint/6331

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