FNDC5 expression in Purkinje neurons of adult male rats with acute spinal cord injury following treatment with methylprednisolone

Hassanzadeh, S. and Jameie, S.B. and Mehdizadeh, M. and Soleimani, M. and Namjoo, Z. and Soleimani, M. (2018) FNDC5 expression in Purkinje neurons of adult male rats with acute spinal cord injury following treatment with methylprednisolone. Neuropeptides, 70. pp. 16-25.

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Abstract

Spinal cord injury (SCI) is a serious and complex medical condition that can happen to anyone. At present, therapy mainly focuses on rehabilitation and pharmacological treatment, such as methylprednisolone (MP). Supra-spinal changes in certain structures, such as the cerebellum, that receive many afferents from the spinal cord might be one reason for unsuccessful therapeutic outcomes. Recently, the expression of FNDC5 was reported in cerebellar Purkinje cells as a possible neuroprotective agent. In the present study, we considered the expression of FNDC5 in Purkinje cells following SCI with and without MP administration in adult rats with SCI. Thirty-five adult male rats were used in this study. The animals were randomly allocated into five groups, including SCI, spinal cord injury with methylprednisolone treatment (SCI + MP), operation sham, control, and operation sham with MP. Induction of SCI was achieved by using special clips to compress the spinal cord at a determined level. After a certain interval time, the animals underwent study for FNDC5 expression, apoptosis by using immunohistochemistry, Western blotting, and TUNEL and Nissl staining. Our results showed a significant decrease in the number of Purkinje cells following SCI. Therapy with MP inhibits apoptosis in irFNDC5 Purkinje cells and restores them. Expression of FNDC5 significantly increased in SCI and decreased following MP therapy. We also showed other cerebellar cells with FNDC5 immunoreactivity in the two other cerebellar layers that were firstly reported. Since irisin is known as a plasma product of FNDC5, we think it might be a plasma marker following therapeutic efforts for SCI; however, it needs further research. In addition, it is possible that changes in FNDC5 expression in Purkinje cells might be related to neurogenesis in the cerebellum with unknown mechanisms. © 2018 Elsevier Ltd

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: fibronectin; fibronectin type III domain containing protein 5; irisin; methylprednisolone sodium succinate; unclassified drug, adult; animal cell; animal experiment; animal model; animal tissue; apoptosis; Article; cell count; cerebellum cell; controlled study; drug efficacy; drug mechanism; immunohistochemistry; immunoreactivity; male; nervous system development; neuroprotection; Nissl staining; nonhuman; priority journal; protein analysis; protein expression; Purkinje cell; rat; sham procedure; spinal cord compression; spinal cord injury; TUNEL assay; Western blotting; Wistar rat
Subjects: WE Musculoskeletal System
QV Pharmacology
Depositing User: eprints admin
Date Deposited: 24 Dec 2018 07:25
Last Modified: 18 Aug 2019 08:18
URI: http://eprints.iums.ac.ir/id/eprint/6334

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