Evaluation of Mn-superoxide dismutase and catalase gene expression in childhood obesity: Its association with insulin resistance

Mohseni, R. and Sadeghabadi, Z.A. and Goodarzi, M.T. and Teimouri, M. and Nourbakhsh, M. and Razzaghy Azar, M. (2018) Evaluation of Mn-superoxide dismutase and catalase gene expression in childhood obesity: Its association with insulin resistance. Journal of Pediatric Endocrinology and Metabolism, 31 (7). pp. 727-732.

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Abstract

Obesity is associated with oxidative stress. Superoxide dismutase (SOD) is the first line of defense against reactive oxygen species (ROS), eliminating the strong superoxide radical and producing H2O2, which can then be degraded by catalase (CAT). The main objective of this study was to evaluate the gene expression antioxidant enzymes (Mn-SOD and CAT) in peripheral blood mononuclear cells (PBMCs) of obese and normal-weight children, and its association with anthropometric and biochemical parameters. Thirty obese and 30 control subjects between the ages of 8 and 16 years were enrolled in this study. Serum insulin levels were measured using enzyme-linked immunosorbent assay (ELISA), and insulin resistance was calculated using the homeostasis model assessment of insulin resistance (HOMA-IR). Biochemical parameters were also measured. PBMCs of the subjects were separated and Mn-SOD and CAT gene expression was measured using real-time polymerase chain reaction (PCR). Mn-SOD and CAT gene expression was significantly lower in the obese group compared with the control group (p<0.01). Also, a positive correlation was observed between the gene expression of Mn-SOD and CAT and body mass index (BMI), fasting blood sugar, insulin resistance, low density lipoprotein-cholesterol (LDL-C) cholesterol, triglycerides (TG) and systolic blood pressure (SBP). Induction of antioxidants, especially Mn-SOD and CAT, can lead to reduction of oxidative stress and prevent the complications of obesity in children. © 2018 Walter de Gruyter GmbH, Berlin/Boston.

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: catalase; glucose; insulin; low density lipoprotein cholesterol; manganese superoxide dismutase; triacylglycerol, adolescent; Article; biochemistry; body mass; body weight; CAT gene; child; childhood obesity; cholesterol blood level; clinical article; controlled study; correlational study; disease association; enzyme linked immunosorbent assay; gene; gene expression; glucose blood level; homeostasis model assessment; human; human cell; insulin blood level; insulin resistance; Mn SOD gene; peripheral blood mononuclear cell; real time polymerase chain reaction; systolic blood pressure; triacylglycerol blood level
Subjects: WK Endocrine System
WS Pediatrics
Depositing User: eprints admin
Date Deposited: 24 Dec 2018 06:52
Last Modified: 24 Dec 2018 06:53
URI: http://eprints.iums.ac.ir/id/eprint/6356

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