Effect of metformin on germ cell-specific apoptosis, oxidative stress and epididymal sperm quality after testicular torsion/detorsion in rats

Ghasemnejad-Berenji, M. and Ghazi-Khansari, M. and Yazdani, I. and Nobakht, M. and Abdollahi, A. and Ghasemnejad-Berenji, H. and Mohajer Ansari, J. and Pashapour, S. and Dehpour, A.R. (2018) Effect of metformin on germ cell-specific apoptosis, oxidative stress and epididymal sperm quality after testicular torsion/detorsion in rats. Andrologia, 50 (2).

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Abstract

Summary: The study was designed to evaluate the effects of metformin on apoptosis and epididymal sperm quality in a rat testicular ischaemia/reperfusion (I/R) injury model. A total of 72 male rats were divided into four groups (n = 18 for each group): group 1 (sham-operated group), group 2 (metformin group), group 3 (torsion/detorsion T/D + saline) and group 4 (T/D + 300 mg kg�1 metformin). Testicular torsion was achieved by rotating the right testis 720° in a clockwise direction for 1 hr. Tissue malondialdehyde (MDA) level and caspase-3 activity increased and the activities of catalase, superoxide dismutase and glutathione peroxidase decreased in comparison with sham-operated group 4 hr after detorsion (p <.001). In six rats of each group 24 hr after detorsion, histopathological changes and germ cell apoptosis were significantly deteriorated by measuring mean of seminiferous tubule diameters (MSTD) and TUNEL test. Moreover, 30 days after T/D, sperm concentration and motility were examined in six animals per group. Metformin pre-treatment reduced MDA and caspase-3 levels and normalised antioxidant enzyme activities 4 hr after detorsion, and germ cell apoptosis was significantly decreased, and the MSTD, as well as sperm functions, was significantly improved. Reduction in oxidative stress and apoptosis may have a major role in cytoprotective effects of metformin. © 2017 Blackwell Verlag GmbH

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: caspase 3; catalase; glutathione peroxidase; malonaldehyde; metformin; sodium chloride; superoxide dismutase; Casp3 protein, rat; caspase 3; catalase; enzyme activator; hydroxymethylglutaryl coenzyme A reductase kinase; malonaldehyde; metformin, animal experiment; animal model; animal tissue; apoptosis; Article; cell protection; controlled study; down regulation; drug effect; enzyme activity; epididymis; germ cell; histopathology; lipid peroxidation; male; nonhuman; oxidative stress; rat; reperfusion injury; semen analysis; seminiferous tubule; single drug dose; sperm quality; spermatozoon density; spermatozoon motility; testis tissue; testis torsion; tissue level; TUNEL assay; upregulation; animal; apoptosis; complication; cytology; disease model; drug effect; epididymis; human; metabolism; oxidative stress; pathology; pathophysiology; physiology; reperfusion injury; spermatozoon; spermatozoon count; testis; testis torsion; Wistar rat, AMP-Activated Protein Kinases; Animals; Apoptosis; Caspase 3; Catalase; Disease Models, Animal; Enzyme Activators; Epididymis; Humans; In Situ Nick-End Labeling; Male; Malondialdehyde; Metformin; Oxidative Stress; Rats; Rats, Wistar; Reperfusion Injury; Sperm Count; Spermatic Cord Torsion; Spermatozoa; Testis
Subjects: WJ Urogenital System
Depositing User: eprints admin
Date Deposited: 12 Dec 2018 14:25
Last Modified: 12 Dec 2018 14:25
URI: http://eprints.iums.ac.ir/id/eprint/6853

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