Molecular characterization of carbapenem-resistant acinetobacter baumannii isolated from pediatric burns patients in an Iranian hospital

Pournajaf, A. and Rajabnia, R. and Razavi, S. and Solgi, S. and Ardebili, A. and Yaghoubi, S. and Khodabandeh, M. and Yahyapour, Y. and Emadi, B. and Irajian, G. (2018) Molecular characterization of carbapenem-resistant acinetobacter baumannii isolated from pediatric burns patients in an Iranian hospital. Tropical Journal of Pharmaceutical Research, 17 (1). pp. 135-141.

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Abstract

Purpose: To survey the molecular characteristics of imipenem-resistant Acinetobacter baumannii obtained from pediatric burns patients in a teaching hospital in Tehran, Iran. Methods: Over a 10-month period, 73 non-duplicate A. baumannii strains were collected from pediatric burns patients admitted to Motahari Burn and Reconstruction Center, Tehran, Iran. The resistance profile of several antimicrobials was determined. Metallo-β-lactamase (MBL)-producing isolates were identified using double-disk synergy and an MBL E-test. Polymerase chain reaction (PCR) was carried out to detect the following β-lactamase-encoding elements: blaVIM, blaIMP, blaSIM, blaSPM, blaGIM, blaNDM, blaAIM, blaDIM, blaKPC, blaOXA-23/24/51, and blaOXA-58. The types of integrons were also identified using PCR. Results: Out of the 73 collected strains, 92.4 and 38.3 of the isolates were multidrug-resistant (MDR) and extensively drug-resistant (XDR), respectively. Colistin was the most effective antibiotic. It was found that 94.5 of the strains were resistant to imipenem, as determined both by disk agar diffusion and MIC E-test methods. Based on double disk synergy and E-test, 78.1 and 83.5 of the isolates, respectively, were MBL producers. The prevalence of blaOXA-23 and blaOXA-24 were 75.4 and 39.1 , respectively. The results also indicate that 62.3, 30.4, and 4.3 of the isolates were positive for blaVIM, blaIMP and blaNDM genes, respectively. Furthermore, 16.4, 76.1, and 7.5 of the isolates carried intI, intII, and intIII genes, respectively. Conclusion: The increased frequency of carbapenem-resistant A. baumannii in burns cases underlines the importance of choosing an appropriate antibacterial regimen based on antibiotic susceptibility profile. Rapid identification of carbapenemase-producing strains would be helpful for selecting suitable antimicrobial therapy and preventing further spread of their encoding genes. © Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, 300001 Nigeria.

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: beta lactamase; ceftazidime; ciprofloxacin; cotrimoxazole; gentamicin; imipenem; levofloxacin; metallo beta lactamase; piperacillin; piperacillin plus tazobactam; tetracycline; tobramycin, Acinetobacter baumannii; adolescent; agar diffusion; antibiotic sensitivity; Article; bacterial gene; bacterium isolation; blaIMP gene; blaNDM gene; blaOXA gene; blaVIM gene; burn patient; carbapenem resistant Acinetobacter baumannii; child; controlled study; disk diffusion; female; human; major clinical study; male; microbiological examination; Modified Hodge test; multidrug resistance; polymerase chain reaction; prevalence
Subjects: WS Pediatrics
QV Pharmacology
Depositing User: eprints admin
Date Deposited: 05 Dec 2018 12:49
Last Modified: 11 Aug 2019 05:58
URI: http://eprints.iums.ac.ir/id/eprint/7118

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