Pretreatment with melatonin protects against cyclophosphamide-induced oxidative stress and renal damage in mice

Goudarzi, M. and Khodayar, M.J. and Hosseini Tabatabaei, S.M.T. and Ghaznavi, H. and Fatemi, I. and Mehrzadi, S. (2017) Pretreatment with melatonin protects against cyclophosphamide-induced oxidative stress and renal damage in mice. Fundamental and Clinical Pharmacology, 31 (6). pp. 625-635.

Full text not available from this repository.
Official URL:


Cyclophosphamide (CP) is widely used in treatment of different cancers. Nephrotoxicity is one of the dose-limiting side effects of CP. This study was carried out to investigate the effect of melatonin (MEL) on CP-induced nephrotoxicity in mice. In this study, 50 Swiss albino mice (20�25 g) were randomly divided into five groups. Mice were pretreated with MEL intraperitoneally (i.p) in doses of 5, 10 and 20 mg/kg for five consecutive days, and CP (200 mg/kg, i.p) was administrated on the 5th day 1 h after the last dose of MEL. Then on day 6, blood samples were collected to determine serum creatinine (Cr) and blood urea nitrogen (BUN) levels. The kidneys were used for histological examination, biochemical assays and real-time PCR studies. Malondialdehyde (MDA), glutathione (GSH), protein carbonyl (PC), nitric oxide (NO) level, catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx) and myeloperoxidase (MPO) activity were assessed in renal tissue. In addition, the expression of SOD2 and PGx1 was measured using real-time PCR method in renal tissue. Results showed that CP administration significantly increases Cr, BUN, MDA, PC, NO level and MPO activity. It also decreases renal GSH level, SOD, GPx and CAT activity. Pretreatment with MEL (especially 20 mg/kg, i.p.) for 5 days prevented these changes; however, it did not affect the SOD activity. Our results revealed that MEL might be useful for prevention of the nephrotoxicity induced by CP through ameliorative effects on biochemical indices and oxidative stress parameters. © 2017 Société Française de Pharmacologie et de Thérapeutique

Item Type: Article
Additional Information: cited By 13
Uncontrolled Keywords: catalase; creatinine; cyclophosphamide; glutathione; glutathione peroxidase; malonaldehyde; manganese superoxide dismutase; melatonin; myeloperoxidase; nitric oxide; protein; protein carbonyl; superoxide dismutase; unclassified drug; alkylating agent; antioxidant; cyclophosphamide; melatonin, animal experiment; animal model; animal tissue; Article; assay; biochemical assay; blood sampling; controlled study; creatinine blood level; drug efficacy; enzyme activity; gene expression; histology; histopathology; kidney function; kidney injury; kidney tissue; lipid peroxidation; male; mouse; nonhuman; oxidative stress; priority journal; real time polymerase chain reaction; renal protection; urea nitrogen blood level; animal; chemically induced; disease model; drug administration; inbred mouse strain; intraperitoneal drug administration; kidney disease; randomization, Animals; Antineoplastic Agents, Alkylating; Antioxidants; Cyclophosphamide; Disease Models, Animal; Drug Administration Schedule; Injections, Intraperitoneal; Kidney Diseases; Male; Melatonin; Mice; Mice, Inbred Strains; Oxidative Stress; Random Allocation
Subjects: WJ Urogenital System
Depositing User: eprints admin
Date Deposited: 29 Dec 2018 10:43
Last Modified: 21 Aug 2019 08:23

Actions (login required)

View Item View Item