λ phage nanobioparticle expressing apoptin efficiently suppress human breast carcinoma tumor growth in vivo

Shoae-Hassani, A. and Keyhanvar, P. and Seifalian, A.M. and Mortazavi-Tabatabaei, S.A. and Ghaderi, N. and Issazadeh, K. and Amirmozafari, N. and Verdi, J. (2013) λ phage nanobioparticle expressing apoptin efficiently suppress human breast carcinoma tumor growth in vivo. PLoS ONE, 8 (11).

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Abstract

Using phages is a novel field of cancer therapy and phage nanobioparticles (NBPs) such as λ phage could be modified to deliver and express genetic cassettes into eukaryotic cells safely in contrast with animal viruses. Apoptin, a protein from chicken anemia virus (CAV) has the ability to specifically induce apoptosis only in carcinoma cells. We presented a safe method of breast tumor therapy via the apoptin expressing λ NBPs. Here, we constructed a λ ZAP-CMV-apoptin recombinant NBP and investigated the effectiveness of its apoptotic activity on BT-474, MDA-MB-361, SKBR-3, UACC-812 and ZR-75 cell lines that over-expressing her-2 marker. Apoptosis was evaluated via annexin-V fluorescent iso-thiocyanate/propidium iodide staining, flow-cytometric method and TUNEL assay. Transfection with NBPs carrying λ ZAP-CMV-apoptin significantly inhibited growth of all the breast carcinoma cell lines in vitro. Also nude mice model implanted BT-474 human breast tumor was successfully responded to the systemic and local injection of untargeted recombinant λ NBPs. The results presented here reveal important features of recombinant λ nanobioparticles to serve as safe delivery and expression platform for human cancer therapy. © 2013 Shoae-Hassani et al.

Item Type: Article
Additional Information: cited By 11
Uncontrolled Keywords: Animals; Antineoplastic Agents; Bacteriophage lambda; Breast Neoplasms; Capsid Proteins; Cell Division; Female; Flow Cytometry; Humans; Mice; Mice, Nude; Nanoparticles; Recombinant Proteins
Subjects: QV Pharmacology
Depositing User: somayeh pourmorteza
Date Deposited: 29 May 2019 13:05
Last Modified: 29 May 2019 13:05
URI: http://eprints.iums.ac.ir/id/eprint/9547

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