Effect of chronic lithium administration on endothelium-dependent relaxation of rat mesenteric bed: Role of nitric oxide

Afsharimani, B. and Moezi, L. and Sadeghipour, H. and Rahimzadeh-Rofouyi, B. and Nobakht, M. and Sanatkar, M. and Ghahremani, M.H. and Dehpour, A.R. (2007) Effect of chronic lithium administration on endothelium-dependent relaxation of rat mesenteric bed: Role of nitric oxide. Canadian Journal of Physiology and Pharmacology, 85 (10). pp. 1038-1046.


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The mechanism of action of lithium, an effective treatment for bipolar disease, is still unknown. In this study, the mesenteric vascular beds of control rats and rats that were chronically treated with lithium were prepared by the McGregor method, and the mesenteric vascular bed vasorelaxation responses were examined. NADPH-diaphorase histochemistry was used to determine the activity of NOS (nitric oxide synthase) in mesenteric vascular beds. We demonstrated that ACh-induced vasorelaxation increased in the mesenteric vascular bed of rats treated with lithium. Acute No-nitro-L-arginine methyl ester (L-NAME) administration in the medium blocked ACh-induced vasorelaxation in the control group more effectively than in lithium-treated rats, while the vasorelaxant response to sodium nitroprusside, a NO donor, was not different between lithium-treated and control groups. Acute aminoguanidine administration blocked ACh-induced vasorelaxation of lithium-treated rats, but had no effect in the control rats. Furthermore, NOS activity, determined by NADPH-diaphorase staining, was significantly greater in the mesenteric vascular beds from chronic lithium-treated rats than in those from control rats. These data suggest that the enhanced ACh-induced endothelium-derived vasorelaxation in rat mesenteric bed from chronic lithium-treated rats might be associated with increased NOS activity, likely via iNOS. Simultaneous acute L-NAME and indomethacin administration suggests the possible upregulation of EDHF (endothelium-derived hyperpolarizing factor) in lithium-treated rats. © 2007 NRC.

Item Type: Article
Additional Information: cited By 7
Uncontrolled Keywords: acetylcholine; aminoguanidine; endothelium derived hyperpolarizing factor; indometacin; lithium; lithium chloride; n(g) nitroarginine methyl ester; nitric oxide synthase; nitroprusside sodium; phenylephrine; prostaglandin; prostaglandin synthase inhibitor; reduced nicotinamide adenine dinucleotide phosphate dehydrogenase, animal experiment; animal model; article; blood vessel reactivity; controlled study; drug blood level; drug effect; drug mechanism; enzyme activity; histochemistry; male; mesentery blood flow; nonhuman; priority journal; rat; vascular endothelium; vasoconstriction; vasodilatation, Animals; Antimanic Agents; Biological Factors; Endothelium, Vascular; Enzyme Inhibitors; Lithium Chloride; Male; Mesenteric Artery, Superior; NADPH Dehydrogenase; NG-Nitroarginine Methyl Ester; Nitric Oxide; Nitric Oxide Donors; Nitric Oxide Synthase Type II; Rats; Rats, Sprague-Dawley; Vasoconstrictor Agents; Vasodilation; Vasodilator Agents
Subjects: QV Pharmacology
Divisions: School of Rehabilitation Sciences
Depositing User: parto mrs bakhtminoo
Date Deposited: 26 Feb 2019 08:53
Last Modified: 26 Feb 2019 08:53
URI: http://eprints.iums.ac.ir/id/eprint/10666

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