Mehdizadeh, A. and Pandesh, S. and Shakeri-Zadeh, A. and Kamrava, S.K. and Habib-Agahi, M. and Farhadi, M. and Pishghadam, M. and Ahmadi, A. and Arami, S. and Fedutik, Y. (2014) The effects of folate-conjugated gold nanorods in combination with plasmonic photothermal therapy on mouth epidermal carcinoma cells. Lasers in Medical Science, 29 (3). pp. 939-948.
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The effects of folate-conjugated gold nanorods in combination with plasmonic photothermal therapy on mouth epidermal carcinoma cells.pdf Download (740kB) | Preview |
Abstract
The use of lasers has emerged to be highly promising for cancer therapy modalities, most commonly, the photothermal therapy method. Unfortunately, the most common disadvantage of laser therapy is its nonselectivity and requirement of high power density. The use of plasmonic nanoparticles as highly enhanced photoabsorbing agents has thus introduced a much more selective and efficient cancer therapy strategy. In this study, we aimed to demonstrate the selective targeting and destruction of mouth epidermal carcinoma cells (KB cells) using the photothermal therapy of folate-conjugated gold nanorods (F-GNRs). Considering the beneficial characteristics of GNRs and overexpression of the folate receptor by KB cells, we selected F-GNRs as a targeted photothermal therapy agent. Cell viability was evaluated using a 3-(4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide assay. Apoptosis was determined by flow cytometry using an annexin V-fluorescein isothiocyanate/propidium iodide apoptosis detection kit. No cell damage or cytotoxicity from the individual treatment of laser light or F-GNRs was observed. However, a 56 cell lethality was achieved for KB cells using combined plasmonic photothermal therapy of 20 μM F-GNRs with seven pulses of laser light and 6-h incubation periods. Cell lethality strongly depends on the concentration of F-GNRs and the incubation period that is mainly due to the induction of apoptosis. This targeted damage is due to the F-GNRs present in the cancer cells strongly absorbing near-infrared laser light and rapidly converting it to heat. This new therapeutic avenue for cancer therapy merits further investigation using in vivo models for application in humans. © 2013 Springer-Verlag.
| Item Type: | Article |
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| Additional Information: | cited By 29 |
| Uncontrolled Keywords: | folate receptor; folic acid; gold nanoparticle; lipocortin 5; nanorod; folic acid; gold; nanotube, absorption; adult; apoptosis; article; carcinoma cell; cell viability; conjugation; controlled study; cytotoxicity; flow cytometry; human; human cell; in vitro study; light exposure; low level laser therapy; male; MTT assay; phototherapy; plasmonic photothermal therapy; priority journal; protein expression; cell death; cell survival; chemistry; drug effects; epidermis; hyperthermic therapy; infrared radiation; laser; metabolism; Mouth Neoplasms; pathology; radiation response; tumor cell line; ultraviolet spectrophotometry, Annexin A5; Cell Death; Cell Line, Tumor; Cell Survival; Epidermis; Folic Acid; Gold; Humans; Hyperthermia, Induced; Infrared Rays; Lasers; Mouth Neoplasms; Nanotubes; Phototherapy; Spectrophotometry, Ultraviolet |
| Subjects: | WU Dentistry. Oral Surgery |
| Depositing User: | Ms Roya Vesal Azad |
| Date Deposited: | 26 Feb 2019 09:16 |
| Last Modified: | 26 Feb 2019 09:16 |
| URI: | http://eprints.iums.ac.ir/id/eprint/11797 |
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