Human olfactory stem cells: As a promising source of dopaminergic neuron-like cells for treatment of Parkinson's disease

Alizadeh, R. and Kamrava, S.K. and Bagher, Z. and Farhadi, M. and Falah, M. and Moradi, F. and Boroujeni, M.E. and Soleimani, M. and Kamyab, A. and Komeili, A. (2019) Human olfactory stem cells: As a promising source of dopaminergic neuron-like cells for treatment of Parkinson's disease. Neuroscience Letters, 696. pp. 52-59.

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Abstract

The production of dopaminergic (DA) neurons from stem cells holds a great promise for future clinical treatment of neurodegenerative diseases, such as Parkinson's disease (PD). Olfactory ecto-mesenchymal stem cells (OE-MSCs) derived from the adult human olfactory mucosa can be easily isolated and expanded in culture while maintaining their immense plasticity. In addition to reduced ethical concerns, OE-MSCs could be transplanted across allogeneic barriers, making them desirable stem cells for clinical applications. The goal of this study was to define the potentiality of human olfactory mucosa-derived MSCs aimed at differentiation into DA neuron-like cells. OE-MSCs were induced to differentiate to DA neuron-like cells in vitro by using sonic hedgehog (SHH), fibroblast growth factor 8 (FGF8), basic fibroblast growth factor (bFGF), Glial cell line-derived neurotrophic factor (GDNF) and brain derived neurotrophic factor (BDNF). Then the differentiated neurons were characterized for expression of DA neuron markers by Real-time PCR, immunocytochemistry and flow cytometry. Our findings showed that differentiated OE-MSCs could significantly express DA neuron markers at mRNA and protein levels along with dopamine release 12 days post-differentiation. These results support the viability and feasibility of using OE-MSCs as a source of in vitro generated DA neuron-like cells for treatment of DA disorders namely PD. © 2018

Item Type: Article
Additional Information: cited By 3
Subjects: WL Nervous System
Depositing User: eprints admin
Date Deposited: 31 Oct 2020 09:35
Last Modified: 31 Oct 2020 09:35
URI: http://eprints.iums.ac.ir/id/eprint/14833

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