Hasanzadeh, A. and Radmanesh, F. and Kiani, J. and Bayandori, M. and Fatahi, Y. and Aref, A.R. and Karimi, M. (2019) Photoluminescent functionalized carbon dots for CRISPR delivery: Synthesis, optimization and cellular investigation. Nanotechnology, 30 (13).
Full text not available from this repository.Abstract
Gene therapy using clustered regularly interspaced short palindromic repeat plasmids (pCRISPR) reduces mistakes in gene editing and prevents engendering integrational mutagenesis that has been seen in available genome engineering technologies. Developing an ideal and traceable nanocarrier, which can accurately and efficiently transfer this complex into the cytosol and which facilitates the journey towards the nucleus, is a fascinating area of research. Polyethylenimine (PEI) functionalized carbon dots (CD-PEI) were fabricated by one-step microwave assisted pyrolysis with an average size around 3 nm. This CD-PEI showed good potential for intracellular delivery of genetic materials (�70). Also, this CD-PEI with passive surface modification with low molecular PEI (2 kDa) has a very high quantum yield, as high as 40 with low cytotoxicity. The expression rate of the pCRISPR was around 15 in the HEK-293 cell which is comparable with the pristine PEI. Furthermore, the CD-PEI demonstrated good properties, such as high quantum yield, biocompatibility and tunable emission wavelengths, suggesting the potential application of photoluminescent functionalized CDs as a suitable, traceable nanocarrier for CRISPR delivery. © 2019 IOP Publishing Ltd.
| Item Type: | Article |
|---|---|
| Additional Information: | cited By 0 |
| Subjects: | QW Microbiology. Immunology |
| Depositing User: | eprints admin |
| Date Deposited: | 26 Oct 2020 10:36 |
| Last Modified: | 26 Oct 2020 10:36 |
| URI: | http://eprints.iums.ac.ir/id/eprint/14998 |
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