Mobini, G.R. and Ghahremani, M.H. and Amanpour, S. and Dehpour, A.R. and Akbari, A. and Hoseiniharouni, S.M. and Muhammadnejad, S. and Sheikhzade, M. and Abedkhojasteh, H. and Mohebi, M. and Bolhassani, M. and Heidari, M. (2016) Transforming growth factor beta-induced factor 2-linked x (TGIF2LX) regulates two morphogenesis genes, Nir1 and Nir2 in human colorectal. Acta Medica Iranica, 54 (5). pp. 302-307.
|
Text
Transforming growth factor beta-induced factor 2-linked x (TGIF2LX) regulates two morphogenesis genes, Nir1 and Nir2 in human colorectal.pdf Download (445kB) | Preview |
Abstract
A member of homeodomain protein namely TGIF2LX has been implicated as a tumor suppressor gene in human malignancy as well as in spermatogenesis. However, to our knowledge, dynamic functional evidence of the TGIF2LX has not yet been provided. The aim of the present study was to investigate the human TGIF2LX target gene(s) using a cDNA-AFLP as a differential display method. A pEGFP-TGIF2LX construct containing the wild-type TGIF2LX cDNA was stably transfected into SW48 cells. UV microscopic analysis and Real-time RT-PCR were used to confirm TGIF2LX expression. The mRNA expressions of TGIF2LX in transfected SW48 cells, the cells containing empty vector (pEGFP-N), and untransfected cells were compared. Also, a Real-time PCR technique was applied to validate cDNA-AFLP results. The results revealed a significant down-regulation and up-regulationby TGIF2LX of Nir1 and Nir2 genes, respectively. The genes are engaged in the cell morphogenesis process. Our findings may provide new insight into the complex molecular pathways underlying colorectal cancer development. © 2016 Tehran University of Medical Sciences. All rights reserved.
| Item Type: | Article |
|---|---|
| Additional Information: | cited By 11 |
| Uncontrolled Keywords: | complementary DNA; double stranded DNA; homeodomain protein; plasmid vector; small interfering RNA; transforming growth factor beta; transforming growth factor beta induced factor 2 linked x; unclassified drug; homeodomain protein; transforming growth factor beta, amplified fragment length polymorphism; Article; cell line; colon adenocarcinoma; controlled study; differential display; DNA synthesis; down regulation; fluorescence microscopy; gene expression; genetic transfection; human; human cell; molecular cloning; morphogenesis; Nir1 gene; Nir2 gene; polymerase chain reaction; proto oncogene; real time polymerase chain reaction; RNA extraction; sequence analysis; SW48 cell line; transforming growth factor beta induced factor 2 linked x gene; tumor suppressor gene; upregulation; colorectal tumor; genetics; morphogenesis; tumor cell line, Amplified Fragment Length Polymorphism Analysis; Cell Line, Tumor; Colorectal Neoplasms; Down-Regulation; Homeodomain Proteins; Humans; Morphogenesis; Real-Time Polymerase Chain Reaction; Transfection; Transforming Growth Factor beta |
| Subjects: | WI Digestive System |
| Depositing User: | eprints admin |
| Date Deposited: | 06 Oct 2020 04:43 |
| Last Modified: | 06 Oct 2020 04:43 |
| URI: | http://eprints.iums.ac.ir/id/eprint/20924 |
Actions (login required)
 |
View Item |
