Iron overload induced apoptic cell death in isolated rat hepatocytes mediated by reactive oxygen species

Allameh, A. and Amini-Harandi, A. and Osati-Ashtiani, F. and O'Brien, P.J. (2008) Iron overload induced apoptic cell death in isolated rat hepatocytes mediated by reactive oxygen species. Iranian Journal of Pharmaceutical Research, 7 (2). pp. 115-121.

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Isolated rat hepatocytes in culture were incubated with different concentrations of iron-sorbitol (50, 100, 150, and 200 μM) to assess the changes in reactive oxygen species (ROS) and lipid peroxidation leading to apoptotic hepatocyte cell death. The viability of hepatocytes was declined depending on the iron concentration. One hour incubation of the cells with 100 μM iron resulted in decreased of the hepatocyte viability down to 50 (EC50 μM). Cellular glutathione (GSH) was depleted depending on the concentration of iron added to the hepatocytes in culture. Decline in cellular GSH was associated with elevation in reactive oxygen species (ROS) generation and formation of thiobarbituric acid reactive substances (TBARS) as index of lipid peroxidation. TBARS concentration was elevated in hepatocytes exposed to >100 μM of iron for 40 min. A significant increase in ROS formation was also observed in cells incubated with 75 μM of iron for 60 and 120 min. The consequences of ROS-mediated damages to hepatocytes were observed by DNA fragmentation, nuclear staining by propidium iddide and finally with induction of apoptotic hepatocyte cell death. Terminal deoxynucleotie transferase-mediated dUTP nick end labeling i.e. TUNEL assay (In situ-cell death-detection kit) and nuclear staining were also used to confirm apoptosis. These data clearly show that iron overload can cause apoptotic cell death in isolated hepatocytes and generation of ROS precedes other changes related to oxidative stress. Copyright © 2008 by School of Pharmacy Shaheed Beheshti University of Medical Sciences and Health Services.

Item Type: Article
Additional Information: cited By 11
Uncontrolled Keywords: glutathione; iron sorbitex; reactive oxygen metabolite; thiobarbituric acid reactive substance, animal cell; apoptosis; article; cell viability; concentration response; controlled study; DNA fragmentation; incubation time; iron overload; lipid peroxidation; liver cell; liver cell culture; liver cell damage; liver toxicity; male; nick end labeling; nonhuman; oxidative stress; rat; staining
Subjects: WI Digestive System
QU Biochemistry. Cell Biology and Genetics
Depositing User: Arezoo Ghasemi siani
Date Deposited: 14 Dec 2020 07:20
Last Modified: 14 Dec 2020 07:20

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