Aminzadeh, A. and Maroof, N.T. and Mehrabani, M. and Juybari, K.B. and Sharifi, A.M. (2020) Investigating the alterations of oxidative stress status, antioxidant defense mechanisms, MAP kinase and mitochondrial apoptotic pathway in adipose-derived mesenchymal stem cells from STZ diabetic rats. Cell Journal, 22. pp. 38-48.
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Investigating-the-alterations-of-oxidative-stress-status-antioxidant-defense-mechanisms-MAP-kinase-and-mitochondrial-apoptotic-pathway-in-adiposederived-mesenchymal-stem-cells-from-STZ-diabetic-rats2020Cell-Jo.pdf Download (1MB) | Preview |
Abstract
Objective: This study aimed to investigate the reliability of diabetic adipose-derived stem cells (ADSCs) for autologous cell-based therapies by exploring the functionality of signalling pathways involved in regulating oxidative stress and apoptosis. Materials and Methods: In this experimental study, ADSCs were isolated from streptozotocin (STZ)-induced diabetic rats (dADSCs) and normal rats (nADSCs). The colonies derived from dADSCs and nADSCs were compared by colony-forming unit (CFU) assay. Reactive oxygen species (ROS) formation and total antioxidant power (TAP) were also measured. Furthermore, the expression of antioxidant enzymes, including catalase (Cat), superoxide dismutase (Sod)-1 and -3, glutathione peroxidase (Gpx)-1, -3 and -4 was measured at mRNA level by semi-quantitative reverse transcriptase polymerase chain reaction assay. The expression of Bax, Bcl2, caspase-3, total and phosphorylated c-Jun N-terminal kinase (JNK) and P38 Mitogen-Activated Protein Kinase (MAPK) at protein level was analyzed by western blotting. Results: The results of this study indicated that viability and plating efficiency of dADSCs were significantly lower than those of nADSCs. ROS generation and TAP level were respectively higher and lower in dADSCs. The gene expression of antioxidant enzymes, including Cat, Sod-1, Gpx-3 and Gpx-4 in dADSCs was significantly greater than that in nADSCs. However, Sod-3 and Gpx-1 mRNA levels were decreased in dADSCs. Moreover, Bax/Bcl-2 protein ratio, caspase-3 protein expression and phosphorylation of JNK and P38 proteins were increased in dADSCs compared to nADSCs. Conclusion: Taken together, diabetes might impair the cellular functions of dADSCs as candidates for autologous cellbased therapies. This impairment seems to be mediated by JNK, P38 MAPKs, and mitochondria pathway of apoptosis and partly by disruption of antioxidant capacity. © 2020 Royan Institute (ACECR). All rights reserved.
| Item Type: | Article |
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| Additional Information: | cited By 0 |
| Uncontrolled Keywords: | antioxidant; caspase 3; catalase; copper zinc superoxide dismutase; extracellular superoxide dismutase; glutathione peroxidase 1; glutathione peroxidase 3; messenger RNA; mitogen activated protein kinase; mitogen activated protein kinase p38; phospholipid hydroperoxide glutathione peroxidase; protein Bax; protein bcl 2; reactive oxygen metabolite; streptozocin; stress activated protein kinase; superoxide dismutase, adipose derived stem cell; animal cell; animal experiment; animal model; apoptosis; Article; cell isolation; cell viability; CFU counting; controlled study; enzyme phosphorylation; male; mesenchymal stem cell; mitochondrial dynamics; nonhuman; oxidative stress; protein expression; rat; real time reverse transcription polymerase chain reaction; signal transduction; streptozotocin-induced diabetes mellitus; Western blotting |
| Subjects: | WK Endocrine System QU Biochemistry. Cell Biology and Genetics |
| Depositing User: | eprints admin |
| Date Deposited: | 03 Oct 2020 04:13 |
| Last Modified: | 03 Oct 2020 04:13 |
| URI: | http://eprints.iums.ac.ir/id/eprint/23070 |
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