Separating mouse malignant cell line (EL4) from neonate spermatogonial stem cells utilizing microfluidic device in vitro

Ashtari, B. and Shams, A. and Esmaeilzadeh, N. and Tanbakooei, S. and Koruji, M. and Moghadam, M.J. and Ansari, J.M. and Moghadam, A.J. and Shabani, R. (2020) Separating mouse malignant cell line (EL4) from neonate spermatogonial stem cells utilizing microfluidic device in vitro. Stem Cell Research and Therapy, 11 (1).

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Separating-mouse-malignant-cell-line-EL4-from-neonate-spermatogonial-stem-cells-utilizing-microfluidic-device-in-vitro2020Stem-Cell-Research-and-TherapyOpen-Access.pdf

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Abstract

Background: Some children who have survived cancer will be azoospermic in the future. Performing isolation and purification procedures for spermatogonial stem cells (SSC) is very critical. In this regard, performing the process of decontamination of cancerous cells is the initial step. The major objective of the present study is to separate the malignant EL4 cell line in mice and spermatogonial stem cells in vitro. Methods: The spermatogonial stem cells of sixty neonatal mice were isolated, and the procedure of co-culturing was carried out by EL4 which were classified into 2 major groups: (1) the control group (co-culture in a growth medium) and (2) the group of co-cultured cells which were separated using the microfluidic device. The percentage of cells was assessed using flow cytometry technique and common laboratory technique of immunocytochemistry and finally was confirmed through the laboratory technique of reverse transcription-polymerase chain reaction (RT-PCR). Results: The actual percentage of EL4 and SSC after isolation was collected at two outlets: the outputs for the smaller outlet were 0.12 for SSC and 42.14 for EL4, while in the larger outlet, the outputs were 80.38 for SSC and 0.32 for EL4; in the control group, the percentages of cells were 21.44 for SSC and 23.28 for EL4 (based on t test (p � 0.05)). Conclusions: The present study demonstrates that the use of the microfluidic device is effective in separating cancer cells from spermatogonial stem cells. © 2020 The Author(s).

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: cisplatin, animal cell; animal tissue; antineoplastic activity; Article; cell culture; cell isolation; cell separation; cell viability; controlled study; EL4 cell line; flow cytometry; IC50; immunocytochemistry; in vitro study; laboratory technique; microfluidics; mouse; newborn; nonhuman; priority journal; reverse transcription polymerase chain reaction; spermatogonium
Subjects: QT Physiology
Depositing User: eprints admin
Date Deposited: 16 Sep 2020 05:12
Last Modified: 16 Sep 2020 05:12
URI: http://eprints.iums.ac.ir/id/eprint/23584

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