The uterine immunological changes may be responsible for repeated implantation failure

Amjadi, F. and Zandieh, Z. and Mehdizadeh, M. and Aghajanpour, S. and Raoufi, E. and Aghamajidi, A. and Aflatoonian, R. (2020) The uterine immunological changes may be responsible for repeated implantation failure. Journal of Reproductive Immunology, 138.

Full text not available from this repository.
Official URL:


A significant part of couples in IVF-ICSI cycles experience Repeated Implantation Failure (RIF). Screening of the embryos with new methods like Next Generation Sequencing and arrays showed that even euploid embryos fail to implant. Immunology is a potent window maybe resolve the RIF problem. In this investigation we employed innate and adaptive immune system PCR array to compare the transcriptome profiles of endometrium in unexplained RIF and healthy fertile women. A total of 21 women were enrolled in the present study, 11women with unexplained RIF and 10 healthy fertile women. After RNA extraction and cDNA synthesis PCR array was performed using RT2 profiler PCR array human innate and adaptive immune responses kit (Qiagen, Cat.No: PAHS-052A). PCR Array data analysis identified significantly greater expression of IL6, IFNG, IL17A, IL23A, IFNA1, IFNB1, CD40 L, CCR4, CCR5, CCR6, CXR3, CCL2, IL2, TLR4, IRF3, STAT3, RAG1, IFNAR1 in unexplained RIF women than in controls (P < 0.05). However, expression of IL1B, IL8, NFKB, HLA-A, HLA-E, CD80, CD40 was significantly lower in unexplained RIF group than in controls (P < 0.05). Our results showed that modulation of immune system in RIF patient is shifted to inflammatory responses as pNK cells, Th17 signaling pathway and TLR signaling pathway are activated. So, by stimulation of immune system and initiation of humoral immune responses the panel of immunity and immunotolerance is completely changed in RIF patients comparing normal. It seems that attention to these alterations individually help physician to manage RIF patients better. © 2020 Elsevier B.V.

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: alpha1 interferon; CD40 antigen; chemokine receptor CCR4; chemokine receptor CCR5; chemokine receptor CCR6; chemokine receptor CXCR3; complementary DNA; gamma interferon; interferon beta serine; interferon regulatory factor 3; interleukin 17; interleukin 2; interleukin 23p19; interleukin 6; monocyte chemotactic protein 1; RAG1 protein; STAT3 protein; toll like receptor 4; transcriptome, adaptive immunity; adult; Article; clinical article; controlled study; endometrium; female; human; humoral immunity; immunological tolerance; immunomodulation; innate immunity; nidation; polymerase chain reaction; priority journal; protein expression; RNA extraction; signal transduction; Th17 cell; TLR signaling; treatment failure; uterus function
Subjects: WP Gynecology
WQ Obstetrics
Depositing User: eprints admin
Date Deposited: 12 Sep 2020 07:36
Last Modified: 12 Sep 2020 07:36

Actions (login required)

View Item View Item