Esfandi, F. and Salehnezhad, T. and Taheri, M. and Afsharpad, M. and Hafez, A.A. and Oskooei, V.K. and Ghafouri-Fard, S. (2020) Expression assessment of a panel of long non-coding RNAs in gastric malignancy. Experimental and Molecular Pathology, 113.
Full text not available from this repository.Abstract
Background: Long non-coding RNAs (lncRNAs) have several important functions in the regulation of cell homeostasis and cell fate. Consequently, abnormal transcription of lncRNAs has been correlated with malignant transformation of cells. These human transcripts have been shown to participate in the progression of gastric cancer. Methods: In the current project, we evaluated expression of a panel of lncRNAs including HULC, MALAT1, FAS-AS1, GAS5, PVT1, OIP5-AS1 and THRIL in 30 gastric cancer tissues and paired adjacent non-cancerous tissues (ANCTs) using quantitative real-time PCR. Results: HULC, OIP5-AS1 and THRIL transcription quantities were significantly lower in gastric tumors compared to ANCTs (P values = .02, 0.02 and 0.007, respectively). Relative transcription quantities of HULC, MALAT1, OIP5-AS1, PVT1, FAS-AS1 and THRIL were associated with the site of the primary tumor (P values = .002, 0.003, 0.002, 0.002, 0.002, and 0.001, respectively). Moreover, relative expression levels of PVT1 were associated with history of smoking (P value = .04). Correlations were identified between transcript quantities of these lncRNAs in both tumor samples and ANCTs. Receiver operating characteristic curve assessment demonstrated that THRIL had the highest diagnostic power among the mentioned lncRNAs (area under curve (AUC) = 0.72, P value = .001). HULC and OIP5-AS1 ranked afterwards (AUC values of 0.69 and 0.68; P values = .005 and 0.007, respectively). Conclusion: The current investigation underscores the dysregulation of these transcripts in gastric cancer specimens and suggests a number of these transcripts for further assessments of their suitability as cancer biomarkers. © 2020 Elsevier Inc.
| Item Type: | Article |
|---|---|
| Additional Information: | cited By 1 |
| Uncontrolled Keywords: | FAS AS1 RNA; GAS5 RNA; HULC RNA; long untranslated RNA; MALAT1 RNA; OIP5 AS1 RNA; PVT1 RNA; THRIL RNA; unclassified drug; long untranslated RNA; messenger RNA, adolescent; adult; area under the curve; Article; clinical feature; controlled study; correlational study; female; gene expression; genetic association; human; human tissue; major clinical study; male; real time polymerase chain reaction; receiver operating characteristic; risk factor; RNA transcription; smoking; stomach cancer; gene expression regulation; genetics; metabolism; middle aged; stomach tumor; young adult, Adolescent; Adult; Female; Gene Expression Regulation, Neoplastic; Humans; Male; Middle Aged; RNA, Long Noncoding; RNA, Messenger; Stomach Neoplasms; Young Adult |
| Subjects: | WI Digestive System |
| Depositing User: | eprints admin |
| Date Deposited: | 12 Sep 2020 06:22 |
| Last Modified: | 12 Sep 2020 06:22 |
| URI: | http://eprints.iums.ac.ir/id/eprint/23821 |
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