Nazari, H. and Heirani-Tabasi, A. and Hajiabbas, M. and Salimi Bani, M. and Nazari, M. and Pirhajati Mahabadi, V. and Rad, I. and Kehtari, M. and Ahmadi Tafti, S.H. and Soleimani, M. (2020) Incorporation of SPION-casein core-shells into silk-fibroin nanofibers for cardiac tissue engineering. Journal of Cellular Biochemistry, 121 (4). pp. 2981-2993.
Full text not available from this repository.Abstract
Mimicking the structure of extracellular matrix (ECM) of myocardium is necessary for fabrication of functional cardiac tissue. The superparamagnetic iron oxide nanoparticles (SPIONs, Fe3O4), as new generation of magnetic nanoparticles (NPs), are highly intended in biomedical studies. Here, SPION NPs (1 wt) were synthesized and incorporated into silk-fibroin (SF) electrospun nanofibers to enhance mechanical properties and topography of the scaffolds. Then, the mouse embryonic cardiac cells (ECCs) were seeded on the scaffolds for in vitro studies. The SPION NPs were studied by scanning electron microscope (SEM), X-ray diffraction (XRD), and transmission electron microscope (TEM). SF nanofibers were characterized after incorporation of SPIONs by SEM, TEM, water contact angle measurement, and tensile test. Furthermore, cytocompatibility of scaffolds was confirmed by 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. SEM images showed that ECCs attached to the scaffolds with elongated morphologies. Also, the real-time PCR and immunostaining studies approved upregulation of cardiac functional genes in ECCs seeded on the SF/SPION-casein scaffolds including GATA-4, cardiac troponin T, Nkx 2.5, and alpha-myosin heavy chain, compared with the ones in SF. In conclusion, incorporation of core-shells in SF supports cardiac differentiation, while has no negative impact on ECCs' proliferation and self-renewal capacity. © 2019 Wiley Periodicals, Inc.
| Item Type: | Article |
|---|---|
| Additional Information: | cited By 2 |
| Uncontrolled Keywords: | casein; homeobox protein Nkx-2.5; myosin heavy chain alpha; nanofiber; silk fibroin; superparamagnetic iron oxide nanoparticle; transcription factor GATA 4; troponin T; water, adult; animal cell; animal experiment; Article; cardiac muscle; cardiac muscle cell; cell proliferation; cell survival; contact angle; electrospinning; embryo; gene expression profiling; heart function; heart tissue; hydrophilicity; immunohistochemistry; in vitro study; morphological trait; mouse; MTT assay; nanoengineering; nonhuman; priority journal; protein expression; real time polymerase chain reaction; scanning electron microscopy; tensile strength; tissue engineering; transmission electron microscopy; upregulation; X ray diffraction |
| Subjects: | QS Human Anatomy QT Physiology |
| Depositing User: | eprints admin |
| Date Deposited: | 12 Sep 2020 05:44 |
| Last Modified: | 12 Sep 2020 05:44 |
| URI: | http://eprints.iums.ac.ir/id/eprint/23834 |
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