Fabrication of chitosan/agarose scaffolds containing extracellular matrix for tissue engineering applications

Saeedi Garakani, S. and Khanmohammadi, M. and Atoufi, Z. and Kamrava, S.K. and Setayeshmehr, M. and Alizadeh, R. and Faghihi, F. and Bagher, Z. and Davachi, S.M. and Abbaspourrad, A. (2020) Fabrication of chitosan/agarose scaffolds containing extracellular matrix for tissue engineering applications. International Journal of Biological Macromolecules, 143. pp. 533-545.

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One of the most effective approaches for treatment of cartilage involves the use of porous three-dimensional scaffolds, which are useful for improving not only cellular adhesion but also mechanical properties of the treated tissues. In this study, we manufactured a composite scaffold with optimum properties to imitate nasal cartilage attributes. Cartilage extracellular matrix (ECM) was used in order to improve the cellular properties of the scaffolds; while, chitosan and agarose were main materials that are used to boost the mechanical and rheological properties of the scaffolds. Furthermore, we explored the effect of the various weight ratios of chitosan, agarose, and ECM on the mechanical and biomedical properties of the composite scaffolds using the Taguchi method. The resulting composites display a range of advantages, including good mechanical strength, porous morphology, partial crystallinity, high swelling ratio, controlled biodegradability rate, and rheological characteristics. Additionally, we performed the cytotoxicity tests to confirm the improvement of the structure and better cell attachments on the scaffolds. Our findings illustrate that the presence of the ECM in chitosan/agarose structure improves the biomedical characteristics of the final scaffold. In addition, we were able to control the mechanical properties and microstructure of the scaffolds by optimizing the polymers' concentration and their resulting interactions. These results present a novel scaffold with simultaneously enhanced mechanical and cellular attributes comparing to the scaffolds without ECM for nasal cartilage tissue engineering applications. © 2019

Item Type: Article
Additional Information: cited By 9
Uncontrolled Keywords: agarose; chitosan, Article; biodegradability; cell adhesion; concentration (parameter); controlled study; crystallization; cytotoxicity test; DNA determination; extracellular matrix; flow kinetics; human; human tissue; hydrogel; nose cartilage; porosity; tissue engineering
Subjects: QS Human Anatomy
Depositing User: eprints admin
Date Deposited: 02 Sep 2020 06:12
Last Modified: 02 Sep 2020 06:12
URI: http://eprints.iums.ac.ir/id/eprint/24167

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