Siahpoosh, A. and Taleb, A.-M. and Almasi, H. (2016) In vitro evaluation of antioxidant activity and total phenol contents of some extracts from ripe fruits of Phoenix dactylifera var Berhi. International Journal of Pharmacognosy and Phytochemical Research, 8 (11). pp. 1855-1862.
Full text not available from this repository.Abstract
This study was aimed at evaluating polyphenolic compounds and antioxidative capacity of Berhi variety of Date as well as comparing the methanolic extract and methanol-chloroform and methanol-aqueous fractions. The methanolic extract was prepared by maceration method. Concentratrd methanolic extract was suspended in water and extracted by chlorophorm. Water and chlorophorm fractions were dried with freeze drier. In this paper, the antioxidant activity of extracts has been analyzed using DPPH (1,1-diphenyl-2-picrylhydrazyl), ABTS (2,2�-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) free-radical scavenging, FRAP (ferric reducing antioxidant power), Iron chelation and erythrocyte hemolysis assays. In addition total phenolic, flavonoids and oligomeric proanthocyanidins compounds were also analyzed. Quantitative phytochemical studies showed the presence abundant amount of phenolic, flavonoids and oligomeric proanthocyanidins compounds in extracts. The IC50 values of, Met-Aqu, methanolic and Met-Chl extracts for scavenging the DPPH and ABTS radical ranged from 161.64 to 238.95 and 52.3 to 152.40, respectively while that of Iron chelation, erythrocyte hemolysis and EC1 of FRAP assay ranged from 695.38 to 925.94, 147.40 to 418.87 and 0.748 to 2.32 µg/ml. Results indicate that the amount of total phenol, flavonoids and oligomeric proanthocyanidins compounds in the extracts is rated as Met-Aqu> Methanol>Met-Chl. Results of antioxdative, iron chelation, and hemolysis tests indicate an order of MetAqu> Methanol>Met-Chl. Better results of Met-Aqu can be because of its more polyphenolic compound. Results above show that this method of preparing fractions from methanolic extract can cause more polyphenolic compound extraction and improvement of the antioxidative effect. © 2016, International Journal of Pharmacognosy and Phytochemical Research. All rights reserved.
| Item Type: | Article |
|---|---|
| Additional Information: | cited By 0 |
| Depositing User: | eprints admin |
| Date Deposited: | 08 Jul 2018 04:01 |
| Last Modified: | 08 Jul 2018 04:01 |
| URI: | http://eprints.iums.ac.ir/id/eprint/3062 |
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