Cross-linked acellular lung for application in tissue engineering: Effects on biocompatibility, mechanical properties and immunological responses

Shirani, A. and Ganji, F. and Golmohammadi, M. and Hashemi, S.M. and Mozafari, M. and Amoabediny, G. and Karkuki Osguei, N. and Samadikuchaksaraei, A. (2021) Cross-linked acellular lung for application in tissue engineering: Effects on biocompatibility, mechanical properties and immunological responses. Materials Science and Engineering C, 122.

Full text not available from this repository.
Official URL:


The concept of providing tissue engineering scaffolds with natural physical properties and minimal immunogenicity has not been systematically approached for the lungs yet. Here, the rat acellular lung tissue (ALT) was cross-linked to provide either EDC/NHS cross-linked tissue (EDC/NHS-CLT) or tannic acid cross-linked tissue (TA-CLT). Young's modulus revealed that EDC/NHS-CLT had mechanical properties similar to the native lung and culture of lung mesenchymal cells showed a higher potential of cell proliferation on EDC/NHS-CLT versus TA-CLT and ALT. The in vitro immunogenicity tests showed a strong induction of T-cell proliferation by TA-CLT and an attenuated macrophage induction by TA-CLT. Processed rat lungs were implanted xenogenically into the mouse peritoneal cavity and the host-implant interactions showed that tannic acid is not released from TA-CLT in a physiologically effective dose. The profile of peritoneal fluid proinflammatory (TNFα, IL-1β, IL-12p70 and IL-17) and anti-inflammatory (IL-10 and TGFβ1) cytokines, and CD3+ T-lymphocytes and CD11b+ macrophages revealed that apart from induction of high levels of IL-17 during the first week and IL-10 during the second to third weeks after implantation by TA-CLT, other indicators of immune reactions to cross-linked tissues were not significantly different from ALT. Also, a high fibrotic reaction to TA-CLT was observed on the weeks 2�3, but alveolar structures were preserved in EDC/NHS-CLT. Our findings show that by controlled EDC/NHS cross-linking, an acellular lung scaffold could be provided with mechanical properties similar to native lung, which promotes mesenchymal lung cells proliferation and does not stimulate recipient's immune system more than a non-cross-linked tissue. © 2021 Elsevier B.V.

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: Biocompatibility; Biological organs; Biomechanics; Cell proliferation; Crosslinking; Cytology; Elastic moduli; Flavonoids; Macrophages; Rats; Scaffolds (biology); T-cells; Tannins, Alveolar structure; Anti-inflammatories; Immunological response; Mesenchymal cells; Peritoneal cavity; Peritoneal fluid; Proinflammatory; Tissue engineering scaffold, Tissue
Subjects: QS Human Anatomy
Depositing User: eprints admin
Date Deposited: 14 Mar 2021 08:31
Last Modified: 14 Mar 2021 08:31

Actions (login required)

View Item View Item