Wound healing properties and antimicrobial activity of platelet-derived biomaterials

Shariati, A. and Moradabadi, A. and Azimi, T. and Ghaznavi-Rad, E. (2020) Wound healing properties and antimicrobial activity of platelet-derived biomaterials. Scientific Reports, 10 (1).


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We analyzed the potential antibacterial effects of two different PdB against methicillin-resistant S. aureus and P. aeruginosa. The third-degree burn wound healing effects of PdB was also studied. Blood samples were obtained from 10 healthy volunteers and biological assays of the PdB were performed and the antimicrobial activity against MRSA and P. aeruginosa was determined using disk diffusion (DD), broth microdilution (BMD), and time-kill assay methods. 48 Wistar albino rats were burned and infected with MRSA. Two groups were injected PdB, the control groups were treated with plasma and received no treatment respectively. In the next step, the rats were euthanized and skin biopsies were collected and histopathologic changes were examined. The results of DD and BMD showed that both PdB performed very well on MRSA, whereas P. aeruginosa was only inhibited by F-PdB and was less susceptible than MRSA to PdBs. The time-kill assay also showed that F-PdB has an antibacterial effect at 4 hours for two strains. Histopathological studies showed that the treated groups had less inflammatory cells and necrotic tissues. Our data suggest that PdB may possess a clinical utility as a novel topical antimicrobial and wound healing agent for infected burn wounds. © 2020, The Author(s).

Item Type: Article
Additional Information: cited By 6
Uncontrolled Keywords: antiinfective agent; biomaterial; cell extract, animal; burn; chemistry; disk diffusion; drug effect; human; male; methicillin resistant Staphylococcus aureus; microbiology; Pseudomonas aeruginosa; Pseudomonas infection; rat; Staphylococcus infection; thrombocyte; Wistar rat; wound healing; wound infection, Animals; Anti-Bacterial Agents; Biocompatible Materials; Blood Platelets; Burns; Cell Extracts; Disk Diffusion Antimicrobial Tests; Humans; Male; Methicillin-Resistant Staphylococcus aureus; Pseudomonas aeruginosa; Pseudomonas Infections; Rats; Rats, Wistar; Staphylococcal Infections; Wound Healing; Wound Infection
Subjects: WH Hemic and Lymphatic Systems
Depositing User: eprints admin
Date Deposited: 03 May 2021 08:45
Last Modified: 03 May 2021 08:45
URI: http://eprints.iums.ac.ir/id/eprint/33490

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