Lohrasbi, V. and Abdi, M. and Asadi, A. and Rohani, M. and Esghaei, M. and Talebi, M. and Amirmozafari, N. (2020) The effect of improved formulation of chitosan-alginate microcapsules of Bifidobacteria on serum lipid profiles in mice. Microbial Pathogenesis, 149.
Full text not available from this repository.Abstract
Background: Probiotics have been associated with many beneficial effects in human digestive physiology. The aim of this study was to evaluate the effect of improved formulation of chitosan-alginate microcapsules of Bifidobacterium strains on serum triglycerides, cholesterol, HDL, and LDL in mice. Methods: Five approved probiotic strains of Bifidobacterium were tested for anti-proliferative effect and interleukin-8 induction on HT-29 cell lines. Bifidobacterium strains plus five approved Lactobacillus were encapsulated in chitosan-alginate microcapsules and tested for its survival in simulated gastrointestinal conditions. These microcapsules were administered to 4 groups of mice (including 1. Bif (Bifidobacterium strains), 2. Lac (Lactobacillus strains), 3. Bif-Lac (Bifidobacterium plus Lactobacillus strains) and 4. Control) for 8 days. At eighth day, the blood of mice were taken and serum levels of triglycerides, cholesterol, HDL, and LDL of them were determined. Results: All of the Bifidobacterium strains significantly (P < 0.001) reduced secretion of IL-8 in HT-29 cells as well as maximum antiproliferative effects (P < 0.001). In addition, all microcapsules showed impressive survival rate in bile (>94.1) and gastrointestinal (>78.28) conditions (P < 0.05). Only Bif-Lac group displayed significantly lower serum cholesterol and LDL levels than control group (P < 0.05). Besides, all groups indicate statistically significant weight loss of mice during the 8 days in comparison with the control group (P < 0.05). Conclusion: The results of this study showed that the microencapsulated probiotics with alginate and chitosan had an effective mean of delivery of viable bacterial cells and non-pharmacological interventions use to reduce serum cholesterol and LDL levels in in-vivo condition. © 2020
| Item Type: | Article |
|---|---|
| Additional Information: | cited By 0 |
| Uncontrolled Keywords: | alginic acid; chitosan; cholesterol; high density lipoprotein cholesterol; interleukin 8; lipid; low density lipoprotein cholesterol; probiotic agent; triacylglycerol, animal experiment; animal model; antiproliferative activity; Article; bacterial cell; bacterial strain; Bifidobacterium; body weight loss; cell viability; cholesterol blood level; comparative study; controlled study; cytokine release; gastrointestinal disease; high density lipoprotein cholesterol level; HT-29 cell line; in vivo study; Lactobacillus; lipid blood level; lipid fingerprinting; low density lipoprotein cholesterol level; microencapsulation; mouse; nonhuman; priority journal; survival rate; triacylglycerol blood level |
| Subjects: | QV Pharmacology |
| Depositing User: | eprints admin |
| Date Deposited: | 08 May 2021 08:53 |
| Last Modified: | 08 May 2021 08:53 |
| URI: | http://eprints.iums.ac.ir/id/eprint/33532 |
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