The effect of improved formulation of chitosan-alginate microcapsules of Bifidobacteria on serum lipid profiles in mice

Lohrasbi, V. and Abdi, M. and Asadi, A. and Rohani, M. and Esghaei, M. and Talebi, M. and Amirmozafari, N. (2020) The effect of improved formulation of chitosan-alginate microcapsules of Bifidobacteria on serum lipid profiles in mice. Microbial Pathogenesis, 149.

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Background: Probiotics have been associated with many beneficial effects in human digestive physiology. The aim of this study was to evaluate the effect of improved formulation of chitosan-alginate microcapsules of Bifidobacterium strains on serum triglycerides, cholesterol, HDL, and LDL in mice. Methods: Five approved probiotic strains of Bifidobacterium were tested for anti-proliferative effect and interleukin-8 induction on HT-29 cell lines. Bifidobacterium strains plus five approved Lactobacillus were encapsulated in chitosan-alginate microcapsules and tested for its survival in simulated gastrointestinal conditions. These microcapsules were administered to 4 groups of mice (including 1. Bif (Bifidobacterium strains), 2. Lac (Lactobacillus strains), 3. Bif-Lac (Bifidobacterium plus Lactobacillus strains) and 4. Control) for 8 days. At eighth day, the blood of mice were taken and serum levels of triglycerides, cholesterol, HDL, and LDL of them were determined. Results: All of the Bifidobacterium strains significantly (P < 0.001) reduced secretion of IL-8 in HT-29 cells as well as maximum antiproliferative effects (P < 0.001). In addition, all microcapsules showed impressive survival rate in bile (>94.1) and gastrointestinal (>78.28) conditions (P < 0.05). Only Bif-Lac group displayed significantly lower serum cholesterol and LDL levels than control group (P < 0.05). Besides, all groups indicate statistically significant weight loss of mice during the 8 days in comparison with the control group (P < 0.05). Conclusion: The results of this study showed that the microencapsulated probiotics with alginate and chitosan had an effective mean of delivery of viable bacterial cells and non-pharmacological interventions use to reduce serum cholesterol and LDL levels in in-vivo condition. © 2020

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: alginic acid; chitosan; cholesterol; high density lipoprotein cholesterol; interleukin 8; lipid; low density lipoprotein cholesterol; probiotic agent; triacylglycerol, animal experiment; animal model; antiproliferative activity; Article; bacterial cell; bacterial strain; Bifidobacterium; body weight loss; cell viability; cholesterol blood level; comparative study; controlled study; cytokine release; gastrointestinal disease; high density lipoprotein cholesterol level; HT-29 cell line; in vivo study; Lactobacillus; lipid blood level; lipid fingerprinting; low density lipoprotein cholesterol level; microencapsulation; mouse; nonhuman; priority journal; survival rate; triacylglycerol blood level
Subjects: QV Pharmacology
Depositing User: eprints admin
Date Deposited: 08 May 2021 08:53
Last Modified: 08 May 2021 08:53

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