Polymorphisms in the genes encoding surface associated proteins of Clostridioides difficile isolates

Aliramezani, A. and Talebi, M. and Douraghi, M. (2020) Polymorphisms in the genes encoding surface associated proteins of Clostridioides difficile isolates. Infection, Genetics and Evolution, 86.

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Background: Although the diversity of Clostridioides difficile toxins have been extensively studied, little is known about the variation in the surface associated proteins (SAPs) which are important in early steps of bacterial colonization and infection. Here, we examined 65C. difficile isolates to identify polymorphisms in the genes encoding SAPs. Methods: PCR was used to amplify slpA, fliC, fliD, cwp66 and cwp84 genes, followed by sequencing. In addition, the antigenicity and immunogenicity properties of different types of SlpA, FliC, FliD, Cwp66 and Cwp84 proteins were predicted in-silico by VaxiJen and BcePred online servers. Results: The predominant slpA sequence type was gr-01 (42.37), followed by hr-01 (11.86) and 078-01 (10.16). In addition, two new slpA subtypes of smz (smz-09-Ir and smz-010-Ir) and a new slpA sequence type (Ir-01) were identified among the isolates examined. Analysis of the nucleotide sequences of fliC, fliD, cwp66 and cwp84 genes revealed 7, 5,5,3 different sequence types, respectively. Insilico analysis of antigenicity of SAPs showed that FliC had the highest level of antigenicity whereas SlpA and Cwp66 proteins had the highest level of immunogenicity. Conclusions: This study pointed to the nucleotide polymorphism in SAPs of C. difficile isolates and demonstrated noticeable diversity in antigenicity and immunogenicity of these proteins which need to be taken into consideration as promising therapeutic or vaccine targets. © 2020 Elsevier B.V.

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: Cwp66 protein; Cwp84 protein; FliC protein; FliD protein; membrane protein; SlpA protein; surface associated protein; unclassified drug, antigenicity; Article; bacterium isolate; Clostridioides difficile; computer model; controlled study; cwp66 gene; cwp84 gene; fliC gene; fliD gene; gene; genetic polymorphism; immunogenicity; nonhuman; nucleotide sequence; priority journal; slpA gene
Subjects: QZ Pathology
Depositing User: eprints admin
Date Deposited: 08 May 2021 08:54
Last Modified: 08 May 2021 08:54
URI: http://eprints.iums.ac.ir/id/eprint/33533

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