Afrasiabi, S. and Pourhajibagher, M. and Chiniforush, N. and Aminian, M. and Rasi Varaei, S.S. and Bahador, A. (2020) Effects of sub-lethal dose of antimicrobial photodynamic therapy on major virulence traits of Streptococcus mutans. Photodiagnosis and Photodynamic Therapy, 32.
Full text not available from this repository.Abstract
Background: Antimicrobial photodynamic therapy (aPDT) as a complementary step of selective removal of dental caries appears as an ideal choice to help manage caries with minimal interventions. Sub-lethal dose of aPDT (sPDT) impressively effects microbial virulence, although it does not kill microorganisms. Therefore, the present study aimed to investigate the influence of sPDT using diode laser plus chlorophyllin-phycocyanin mixture (PhotoActive+) on changes in gtfB gene expression and the subsequent protein expression of GtfB. Materials and methods: sPDT using PhotoActive+ and 635 ± 10 nm diode laser was used to examine the expression of gtfB mRNA and GtfB protein expression of Streptococcus mutans by quantitative real time PCR (qRT-PCR) method and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), respectively. Results: In this study, sPDT using 2.4 � 10�3 mol/L PhotoActive+ with 3 min irradiation time of diode laser with energy density of 104 J/cm2, significantly reduced the gtfB gene expression with rates of 3.5-fold (P < 0.05). Also, PhotoActive+ mediated sPDT demonstrated a significant reduction in GtfB protein expression of S. mutans up to 54 (P < 0.05). Conclusions: This study demonstrated the potential effect of PhotoActive+ mediated sPDT on one of the most important virulence factors of S. mutans. © 2020 Elsevier B.V.
| Item Type: | Article |
|---|---|
| Additional Information: | cited By 0 |
| Uncontrolled Keywords: | antiinfective agent; chlorophyll; glucosyltransferase; messenger RNA; photosensitizing agent; phycocyanin; virulence factor, Article; bacterial strain; bacterial virulence; bacterium culture; biofilm; dental caries; fluorometry; gene expression; minimum inhibitory concentration; mRNA expression level; nonhuman; photodynamic therapy; polyacrylamide gel electrophoresis; priority journal; protein expression; real time polymerase chain reaction; spectrophotometry; Streptococcus mutans; sublethal dose |
| Subjects: | WN Radiology. Diagnostic Imaging QV Pharmacology |
| Depositing User: | eprints admin |
| Date Deposited: | 08 May 2021 09:18 |
| Last Modified: | 08 May 2021 09:18 |
| URI: | http://eprints.iums.ac.ir/id/eprint/33537 |
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