Ajdary, M. and Zandieh, Z. and Amjadi, F.S. and Keyhanfar, F. and Mehdizadeh, M. and Aflatoonian, R. (2020) Interaction of sperm with endometrium can regulate genes involved in endometrial receptivity pathway in mice: An experimental study. International Journal of Reproductive BioMedicine, 18 (10). pp. 815-824.
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Interaction of sperm with endometrium can regulate genes involved in endometrial receptivity pathway in mice An experimental study.pdf Download (395kB) | Preview |
Abstract
Background: Many researchers consider implantation and endometrial receptivity as pertinent issues in reproductive science. Although, several experiments have been performed and their results evaluated, yet there is no confirmed evidence about the related factors and the role of sperm in endometrial receptivity. Objective: To investigate the effect of the sperm-endometrium interaction in regulating genes involved in the endometrial receptivity pathway. Materials and Methods: In this experimental study, 10 male and 30 female NMRI mice were included, and half of the male cases were vasectomized. The subjects were divided into two groups as follows; group 1 (case) comprised of 15 females mated with 5 non-vasectomized male mice, while group 2 (control) consisted of 15 females mated with 5 vasectomized males. Cases were sacrificed and assessed after 36 hr and the endometrial tissue was extracted and kept at-80°C until the next use. The expression of the endometrial receptivity pathway genes, including VEGF, HBEGF, FGF2, EGF, LIF, LIFR, HOXA10, MUC1, PGR, and CSF, was examined in both groups. For statistical analysis, an independent samples test (Mean ± SD) was used. Results: The mRNA levels of LIF (p = 0.045), LIFR (p = 0.040), MUC1 (p = 0.032), VEGF (p = 0.022), EFG (p = 0.035), and FGF2 (p = 0.040) were significantly upregulated in the case group compared with the control group. Conclusion: Finally, seminal plasma was observed to be effective in expressing the involved genes in the successful implantation pathway, including LIF, LIFR, MUC1, VEGF, EGF, and FGF2. © Ajdary et al.
| Item Type: | Article |
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| Additional Information: | cited By 0 |
| Uncontrolled Keywords: | epidermal growth factor; fibroblast growth factor 2; homeobox protein Hox-A10; ketamine; messenger RNA; mucin 1; vasculotropin; xylazine, animal experiment; animal model; animal tissue; Article; controlled study; CSF gene; down regulation; endometrium; female; gene; gene expression; HBEGF gene; histopathology; LIFR gene; male; mouse; mRNA expression level; nonhuman; PGR gene; protein expression; real time polymerase chain reaction; RNA extraction; semen analysis; spermatozoon motility; upregulation; vasectomy |
| Subjects: | WQ Obstetrics |
| Depositing User: | eprints admin |
| Date Deposited: | 19 Jun 2021 07:33 |
| Last Modified: | 19 Jun 2021 08:44 |
| URI: | http://eprints.iums.ac.ir/id/eprint/33889 |
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