Mohammadi, F. and Ashrafi, M. and Zandieh, Z. and Najafi, M. and Niknafs, B. and Amjadi, F.S. and Haghighi, M. (2020) The effect of preincubation time and myo-inositol supplementation on the quality of mouse mii oocytes. Journal of Reproduction and Infertility, 21 (4). pp. 259-268.
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The effect of preincubation time and myo-inositol supplementation on the quality of mouse mii oocytes.pdf Download (745kB) | Preview |
Abstract
Background: It is demonstrated that optimal preincubation time improves oocyte quality, fertilization potential and developmental rate. This study aimed to evaluate the effect of preincubation time in the simple and myo-inositol supplemented medium on the oocyte quality regarding oxidative stress and mitochondrial alteration. Methods: Cumulus oocyte complexes (COCs) retrieved from superovulated NMRI mice were divided in groups of 0, 4 and 8 hr preincubation time in the simple and 20 mmol/L myo-inositol supplemented media. Intracellular reactive oxygen species (H2O2), glutathione (GSH), mitochondrial membrane potential (MMP), ATP content, and mitochondrial amount were measured and analyzed in experimental groups. One-way ANOVA and Kruskal-Wallis were respectively used for parametric and nonparametric variables. Statistical significance was defined as p<0.05. Results: In comparison to control group, variables including ROS, GSH, mitochondrial amount, fertilization and developmental rates were significantly changed after 4 hr of preincubation in the simple medium, while MMP decreased following 8 hr of preincubation in the simple medium (p<0.001). Preincubation of oocytes up to 8 hr in the simple medium could not decrease ATP content. For both 4 and 8 hr preincubation times, myo-inositole could decrease H2O2 and increase GSH and MMP levels and consequently could improve fertilization rate compared to oocytes preincubated in the simple culture. Conclusion: It seems that 4 hr or more preincubation time can decrease the oocyte quality and lead to reduced oocyte fertilization and developmental potential. Howevere, myo-inositol may prevent oocyte quality reduction and improve fertilization potential in comparision to the equivalent simple groups. © 2020 Avicenna Research Institute. All rights reserved.
| Item Type: | Article |
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| Additional Information: | cited By 0 |
| Uncontrolled Keywords: | adenosine triphosphate; glutathione; hydrogen peroxide; inositol; reactive oxygen metabolite, adult; animal cell; animal experiment; Article; controlled study; female; in vitro fertilization; male; mitochondrial membrane potential; mitochondrion; mouse; nonhuman; oocyte; oxidative stress |
| Subjects: | WQ Obstetrics |
| Depositing User: | eprints admin |
| Date Deposited: | 16 Jun 2021 09:05 |
| Last Modified: | 19 Jun 2021 08:40 |
| URI: | http://eprints.iums.ac.ir/id/eprint/33928 |
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