Fateh, A. and Aghasadeghi, M. and Siadat, S.D. and Vaziri, F. and Sadeghi, F. and Fateh, R. and Keyvani, H. and Tasbiti, A.H. and Yari, S. and Ataei-Pirkooh, A. and Monavari, S.H. (2016) Comparison of Three Different Methods for Detection of IL28 rs12979860 Polymorphisms as a Predictor of Treatment Outcome in Patients with Hepatitis C Virus. Osong Public Health and Research Perspectives, 7 (2). pp. 83-89.
Full text not available from this repository.Abstract
Objectives: This study aimed to evaluate the specificity, sensitivity, cost, and turn-around time of three methods of gene polymorphism analysis and to study the relationship between IL28B rs12979860 and SVR rate to pegIFN-α/RVB therapy among patients with chronic hepatitis C. Methods: A total of 100 samples from chronic hepatitis C patients were analyzed in parallel using the three methods: direct sequencing, real-time polymerase chain reaction (PCR), amplification refractory mutation system (ARMS)-PCR. Results: The different profiles for IL28B rs12979860 alleles (CC, CT, and TT) obtained with PCR-RFLP, ARMS-PCR, and direct sequencing were consistent among the three methods. Prevalence of rs12979860 genotypes CC, CT and TT in HCV genotype 1a was 10(19.6), 35(68.6), and six (11.8), respectively, and in HCV genotype 31, it was 13(26.5), 31(63.3), and five (10.2), respectively. No significant difference was seen between rs12979860 genotype and HCV genotype (p = 0.710). Conclusion: Screening by ARMS - PCR SNOP detection represents the most efficient and reliable method to determine HCV polymorphisms in routine clinical practice. © 2016.
| Item Type: | Article |
|---|---|
| Additional Information: | cited By 11 |
| Depositing User: | eprints admin |
| Date Deposited: | 04 Jul 2018 06:00 |
| Last Modified: | 04 Jul 2018 06:00 |
| URI: | http://eprints.iums.ac.ir/id/eprint/3787 |
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