Darvishi, M. and Hamidabadi, H.G. and Bojnordi, M.N. and saeednia, S. and Zahiri, M. and Niapour, A. and Alizadeh, R. (2021) Differentiation of human dental pulp stem cells into functional motor neuron: In vitro and ex vivo study. Tissue and Cell, 72.
Full text not available from this repository.Abstract
There are several therapeutic options for spinal cord injury (SCI), among these strategies stem cell therapy is a potential treatment. The stem cells based therapies have been investigating in acute phase of clinical trials for promoting spinal repair in humans through replacement of functional neuronal and glial cells. The aim of this study was to evaluate the differentiation of Human Dental Pulp Stem Cells (hDPSCs) into functional motor neuron like cells (MNLCs) and promote neuroregeneration by stimulating local neurogenesis in the adult spinal cord slice culture. The immunocytochemistry analysis demonstrated that hDPSCs were positive for mesenchymal stem cell markers (CD73, CD90 and CD105) and negative for the hematopoietic markers (CD34 and CD45). hDPSCs were induced to neurospheres (via implementing B27, EGF, and bFGF) and then neural stem cells (NSC). The NSC differentiated into MNLCs in two steps: first by Shh and RA and; then with GDNF and BDNF administration. The NS and the NSC were assessed for Oct4, nestin, Nanog, Sox2 expression while the MNLCs were evaluated by ISLET1, Olig2, and HB9 genes. Our results showed that hDPSC can be differentiated into motor neuron phenotype with expression of the motor neuron genes. The functionality of MNLCs was demonstrated by FM1-43, intracellular calcium ion shift and co- culture with C2C12. We co-cultivated hDPSCs with adult rat spinal slices in vitro. Immunostaining and hoechst assay showed that hDPSCs were able to migrate, proliferate and integrate in both the anterolateral zone and the edges of the spinal slices. © 2021 Elsevier Ltd
| Item Type: | Article |
|---|---|
| Additional Information: | cited By 0 |
| Uncontrolled Keywords: | 5' nucleotidase; brain derived neurotrophic factor; calcium ion; CD34 antigen; endoglin; epidermal growth factor; fibroblast growth factor 2; glial cell line derived neurotrophic factor; HLA B27 antigen; nestin; octamer transcription factor 4; oligodendrocyte transcription factor 2; receptor type tyrosine protein phosphatase C; sonic hedgehog protein; Thy 1 membrane glycoprotein; transcription factor NANOG; transcription factor Sox2, adult; aging; animal cell; animal tissue; Article; assay; C2C12 cell line; cell differentiation; cell function; cell migration; cell proliferation; coculture; controlled study; dental pulp stem cell; ex vivo study; gene; gene expression; HB9 gene; hoechst assay; human; human cell; human tissue; immunocytochemistry; immunohistochemistry; in vitro study; ISLET1 gene; male; motoneuron; nerve regeneration; nervous system development; nonhuman; Olig2 gene; protein expression; rat; spinal cord slice; tissue culture; young adult |
| Subjects: | QU Biochemistry. Cell Biology and Genetics |
| Depositing User: | eprints admin |
| Date Deposited: | 31 Aug 2021 04:34 |
| Last Modified: | 31 Aug 2021 04:34 |
| URI: | http://eprints.iums.ac.ir/id/eprint/39014 |
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