Tissue-engineered nerve graft using silk-fibroin/polycaprolactone fibrous mats decorated with bioactive cerium oxide nanoparticles

Saremi, J. and Khanmohammadi, M. and Azami, M. and Ai, J. and Yousefi-Ahmadipour, A. and Ebrahimi-Barough, S. (2021) Tissue-engineered nerve graft using silk-fibroin/polycaprolactone fibrous mats decorated with bioactive cerium oxide nanoparticles. Journal of Biomedical Materials Research - Part A, 109 (9). pp. 1588-1599.

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The main aim of this study was to evaluate the efficacy of cerium oxide nanoparticles (CNPs) encapsulated in fabricated hybrid silk-fibroin (SF)/polycaprolactone (PCL) nanofibers as an artificial neural guidance conduit (NGC) applicable for peripheral nerve regeneration. The NGC was prepared by PCL and SF filled with CNPs. The mechanical properties, contact angle, and cell biocompatibility experiments showed that the optimized concentration of CNPs inside SF and SF/PCL wall of conduits was 1 (wt/wt). The SEM image analysis showed the nanoscale texture of the scaffold in different topologies depend on composition with fiber diameters at about 351 ± 54 nm and 420 ± 73 nm respectively for CNPs + SF and CNPs + SF/PCL fibrous mats. Furthermore, contact angle measurement confirmed the hydrophilic behavior of the membranes, ascribable to the SF content and surface modification through modified methanol treatment. The balance of morphological and biochemical properties of hybrid CNPs 1 (wt/wt) + SF/PCL construct improves cell adhesion and proliferation in comparison with lower concentrations of CNPs in nanofibrous scaffolds. The release of CNPs 1 (wt/wt) from both CNPs + SF and CNPs+ SF/PCL fibrous mats was highly controlled and very slow during the extended time of incubation until 60 days. Fabricated double-layered NGC using CNPs + SF and CNPs + SF/PCL fibers was consistent for application in nervous tissue engineering and regenerative medicine from a structural and biocompatible perspective. © 2021 Wiley Periodicals LLC

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: Biocompatibility; Biomechanics; Cell adhesion; Contact angle; Nanofibers; Nanoparticles; Oxides; Scaffolds (biology); Textures; Tissue, Biochemical properties; Cell biocompatibilities; Cerium oxide nanoparticle; Nanofibrous scaffolds; Neural guidance; Peripheral nerve regeneration; SEM image analysis; Tissue engineered nerve grafts, Cerium oxide
Subjects: QT Physiology
Depositing User: eprints admin
Date Deposited: 01 Sep 2021 06:09
Last Modified: 01 Sep 2021 06:09
URI: http://eprints.iums.ac.ir/id/eprint/39050

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