Olfactory mucosa stem cells delivery via nasal route: a simple way for the treatment of Parkinson disease

Simorgh, S. and Alizadeh, R. and Shabani, R. and Karimzadeh, F. and Seidkhani, E. and Majidpoor, J. and Moradi, F. and Kasbiyan, H. (2021) Olfactory mucosa stem cells delivery via nasal route: a simple way for the treatment of Parkinson disease. Neurotoxicity Research, 39 (3). pp. 598-608.

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Abstract

Finding a simple and effective way for transferring cells to the brain lesion site with minimum side effects mounts a challenge in cell therapy. Cell delivery via nasal route using the bypassing the blood-brain barrier (BBB) property is a simple and non-invasive strategy without serious complications such as trauma. Therefore, it is a suitable technique to treat neurodegenerative disorders like Parkinson�s disease (PD). Olfactory ectomesenchymal stem cells (OE-MSCs) located in the lamina propria of olfactory mucosa could be differentiated into dopaminergic neurons under in vitro and in vivo conditions. Thus, OE-MSCs represent a good source of Parkinson�s stem cell�based therapy. In this research, we studied thirty male rats (n = 10 in each group) in three control (Ctl), lesion (LE), and intranasal administration (INA) groups to investigate the therapeutic effect of intranasal injection of OE-MSCs in the Parkinson�s animal models. To do so, we examined the homing variation of OE-MSCs in different brain regions such as olfactory bulb (OB), cortex, striatum (Str), hippocampus (HPC), and substantia nigra (SN). The results of real-time PCR and immunohistochemistry (IHC) analysis showed the expression of dopaminergic neuron markers such as PITX3, PAX2, PAX5 (as dopaminergic neurons markers), tyrosine hydroxylase (TH), and dopamine transporter (DAT) 2 months after INA of 1 � 106 OE-MSCs. The results confirmed that IN OE-MSCs delivery into the central nervous system (CNS) was powerful enough to improve the behavioral functions in the animal models of PD. Graphical Abstract: Figure not available: see fulltext. © 2021, Springer Science+Business Media, LLC, part of Springer Nature.

Item Type: Article
Additional Information: cited By 2
Subjects: WL Nervous System
QU Biochemistry. Cell Biology and Genetics
Depositing User: eprints admin
Date Deposited: 13 Dec 2021 07:15
Last Modified: 13 Dec 2021 07:15
URI: http://eprints.iums.ac.ir/id/eprint/39343

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