Artificial testis: A testicular tissue extracellular matrix as a potential bio-ink for 3D printing

Bashiri, Z. and Amiri, I. and Gholipourmalekabadi, M. and Falak, R. and Asgari, H. and Maki, C.B. and Moghaddaszadeh, A. and Koruji, M. (2021) Artificial testis: A testicular tissue extracellular matrix as a potential bio-ink for 3D printing. Biomaterials Science, 9 (9). pp. 3465-3484.

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Testicular scaffolds may be an option for fertility preservation. The aim was to develop various procedures for the decellularization of testicular tissue and to design a bio-ink to construct a bioartificial testis. Ram testicular tissue fragments were decellularized using NaCl buffer, NaCl buffer-Triton, SDS and SDS-Triton. The removal of the cells from the tissues was confirmed by DAPI and H & E staining, as well as the evaluation of the DNA content. Alcian blue, Orcein and Masson's trichrome staining methods were also used to confirm that T-ECM was preserved intact. Then, the optimal decellularization protocol was selected to determine the parameters of the bio-ink and printing of the scaffold. The extracted T-ECM was used to print the hydrogel scaffold in combination with alginate-gelatin. The printability, morphological, mechanical and biological properties of the printed hydrogels were characterized. Decellularization of testicular tissue fragments using the NaCl buffer-Triton protocol was significantly more efficient than other decellularization methods in removing the cellular debris and preserving the T-ECM compounds. The 3D printed scaffold with 5 T-ECM showed a uniform surface morphology with high cell attachment and cyto-biocompatibility properties for spermatogonia stem cells in vitro and in vivo compared to other groups. It is concluded that T-ECM can be used as a biomimetic material to make an artificial testis with possible in vitro sperm production. © 2021 The Royal Society of Chemistry.

Item Type: Article
Additional Information: cited By 1
Uncontrolled Keywords: Biocompatibility; Biomimetic materials; Biomimetics; Hydrogels; Morphology; Petroleum reservoir evaluation; Scaffolds (biology); Sodium chloride; Stem cells; Surface morphology; Tissue, Biological properties; Cell attachments; Cellular debris; Decellularization; Decellularized; Extracellular matrices; Hydrogel scaffolds; Staining method, 3D printers, ink, extracellular matrix; male; testis; three dimensional printing; tissue engineering; tissue scaffold, Extracellular Matrix; Ink; Male; Printing, Three-Dimensional; Testis; Tissue Engineering; Tissue Scaffolds
Subjects: WJ Urogenital System
Depositing User: eprints admin
Date Deposited: 21 Dec 2021 10:16
Last Modified: 21 Dec 2021 10:16

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