Bashiri, Z. and Amiri, I. and Gholipourmalekabadi, M. and Falak, R. and Asgari, H. and Maki, C.B. and Moghaddaszadeh, A. and Koruji, M. (2021) Artificial testis: A testicular tissue extracellular matrix as a potential bio-ink for 3D printing. Biomaterials Science, 9 (9). pp. 3465-3484.
Full text not available from this repository.Abstract
Testicular scaffolds may be an option for fertility preservation. The aim was to develop various procedures for the decellularization of testicular tissue and to design a bio-ink to construct a bioartificial testis. Ram testicular tissue fragments were decellularized using NaCl buffer, NaCl buffer-Triton, SDS and SDS-Triton. The removal of the cells from the tissues was confirmed by DAPI and H & E staining, as well as the evaluation of the DNA content. Alcian blue, Orcein and Masson's trichrome staining methods were also used to confirm that T-ECM was preserved intact. Then, the optimal decellularization protocol was selected to determine the parameters of the bio-ink and printing of the scaffold. The extracted T-ECM was used to print the hydrogel scaffold in combination with alginate-gelatin. The printability, morphological, mechanical and biological properties of the printed hydrogels were characterized. Decellularization of testicular tissue fragments using the NaCl buffer-Triton protocol was significantly more efficient than other decellularization methods in removing the cellular debris and preserving the T-ECM compounds. The 3D printed scaffold with 5 T-ECM showed a uniform surface morphology with high cell attachment and cyto-biocompatibility properties for spermatogonia stem cells in vitro and in vivo compared to other groups. It is concluded that T-ECM can be used as a biomimetic material to make an artificial testis with possible in vitro sperm production. © 2021 The Royal Society of Chemistry.
| Item Type: | Article |
|---|---|
| Additional Information: | cited By 1 |
| Uncontrolled Keywords: | Biocompatibility; Biomimetic materials; Biomimetics; Hydrogels; Morphology; Petroleum reservoir evaluation; Scaffolds (biology); Sodium chloride; Stem cells; Surface morphology; Tissue, Biological properties; Cell attachments; Cellular debris; Decellularization; Decellularized; Extracellular matrices; Hydrogel scaffolds; Staining method, 3D printers, ink, extracellular matrix; male; testis; three dimensional printing; tissue engineering; tissue scaffold, Extracellular Matrix; Ink; Male; Printing, Three-Dimensional; Testis; Tissue Engineering; Tissue Scaffolds |
| Subjects: | WJ Urogenital System |
| Depositing User: | eprints admin |
| Date Deposited: | 21 Dec 2021 10:16 |
| Last Modified: | 21 Dec 2021 10:16 |
| URI: | http://eprints.iums.ac.ir/id/eprint/39389 |
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